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Titolo:
Diversity and evolution of a non-TIR-NBS sequence family that clusters to a chromosomal "hotspot" for disease resistance genes in soybean
Autore:
Jeong, SC; Hayes, AJ; Biyashev, RM; Maroof, MAS;
Indirizzi:
Virginia Polytech Inst & State Univ, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA Virginia Polytech Inst & State Univ Blacksburg VA USA 24061 VA 24061 USA
Titolo Testata:
THEORETICAL AND APPLIED GENETICS
fascicolo: 2-3, volume: 103, anno: 2001,
pagine: 406 - 414
SICI:
0040-5752(200108)103:2-3<406:DAEOAN>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROOT-KNOT NEMATODE; NUCLEOTIDE-BINDING; DIVERGENT SELECTION; MARKER ANALYSIS; DEATH PROCESS; LOCI; ANALOGS; LETTUCE; TOMATO; MAIZE;
Keywords:
soybean disease resistance; nucleotide binding site motif; gene family;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Maroof, MAS Virginia Polytech Inst & State Univ, Dept Crop & Soil EnvironmSci, Blacksburg, VA 24061 USA Virginia Polytech Inst & State Univ Blacksburg VA USA 24061 A
Citazione:
S.C. Jeong et al., "Diversity and evolution of a non-TIR-NBS sequence family that clusters to a chromosomal "hotspot" for disease resistance genes in soybean", THEOR A GEN, 103(2-3), 2001, pp. 406-414

Abstract

In soybean, genes controlling resistance to numerous diseases have been shown to cluster to regions on several chromosomes. One such vital chromosomal region is on the soybean molecular linkage group (MLG) F flanked by the RFLP markers K644 and B212. Here, genes controlling resistance to bacterial blight, Phytophthora root rot, and several viral diseases, as well as QTLs conditioning resistance to corn earworm, root knot nematode, and white moldhave been mapped. We have previously identified two classes (b and j) of disease resistance-related nucleotide binding site (NBS) sequences that localize to this cluster of genes. Using both cDNA and genomic analyses, we have studied one multi-gene family of sequences representing the previously reported class j NBS of soybean. This class of NBS resembles the RPS2-like NBS sequences. RPS2 and similar resistance genes are referred to as non-TIR because they do not encode motifs homologous to the Toll-Interleukin-1 region (TIR). By designing PCR primers that specifically target these non-TIR-NBS encoding sequences, we have amplified at least six class j sequence members from soybean. In addition, we have conducted genomic and cDNA library screenings to identify additional class j members. In all, we have characterized 12 class j NBS sequence members. These members have been mapped within a 2-cM region of the soybean F linkage group. We have also identified homoeologous chromosomal regions on linkage groups A2 and E that contain class jNBS sequences. A BLAST search of the GenBank database has shown that non-TIR NBS sequences are present across the legume family. We have compared these non-TIR sequences from other legumes with the soybean clones to assess the level of diversity within this class or disease resistance-related sequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 23:57:11