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Titolo:
Direct visualization of telomeric DNA loops in cells by AFM
Autore:
Chen, A; Cao, EH; Sun, XG; Qin, JF; Liu, D; Wang, C; Bai, CL;
Indirizzi:
Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China Acad Sinica Beijing Peoples R China 100101 ijing 100101, Peoples R China Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China Chinese Acad Sci Beijing Peoples R China 100080 100080, Peoples R China
Titolo Testata:
SURFACE AND INTERFACE ANALYSIS
fascicolo: 1, volume: 32, anno: 2001,
pagine: 32 - 37
SICI:
0142-2421(200108)32:1<32:DVOTDL>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATOMIC-FORCE MICROSCOPY; CHROMOSOMES; MECHANISM; DUPLEX; LONG; ENDS;
Keywords:
telomeres; telomeric DNA; T-loop; G-quartet; atomic force microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Cao, EH Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China Acad Sinica Beijing Peoples R China 100101 0101, Peoples R China
Citazione:
A. Chen et al., "Direct visualization of telomeric DNA loops in cells by AFM", SURF INT AN, 32(1), 2001, pp. 32-37

Abstract

Telomeres are an essential nucleoprotein structure at the ends of all eukaryotic chromosomes. Studies on the structure and function of telomeres havereceived particular attention owing to their possible significance to ageing and cancer. In this work, human telomere from HeLa cells were isolated and purification by bio-gel P-2 column. Large duplex loops with a tail were first observed directly in the purified telomeric DNA species by atomic force microscopy (AFM). The lengths of the loop and tail are similar to2.5 +/-0.5 and 2.0 +/- 1.5 kb, respectively. The AFM images also showed that the circular portion of loops is assumed to be a double-stranded structure according to its apparent height, but to be a triplex or tetraplex structure for the loop-tail junction. These results indicate that telomeres in human cells may end in large loops, which is an advantage in further studies on thestructure and function of telomeres in cells and provides a new procedure for the study of telomere structure. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:31:47