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Titolo:
Visualization of the intermediates in a uniform DNA condensation system bytapping mode atomic force microscopy
Autore:
Liu, D; Wang, C; Lin, Z; Li, JW; Xu, B; Wei, ZQ; Wang, ZG; Bai, CL;
Indirizzi:
Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China Chinese Acad Sci Beijing Peoples R China 100080 100080, Peoples R China
Titolo Testata:
SURFACE AND INTERFACE ANALYSIS
fascicolo: 1, volume: 32, anno: 2001,
pagine: 15 - 19
SICI:
0142-2421(200108)32:1<15:VOTIIA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SINGLE-CRYSTALS; SPERMIDINE; MECHANISM; CATIONS;
Keywords:
AFM; intermediate; DNA; cobalt hexammine; condensation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Bai, CL Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China Chinese Acad Sci Beijing Peoples R China 100080 Peoples R China
Citazione:
D. Liu et al., "Visualization of the intermediates in a uniform DNA condensation system bytapping mode atomic force microscopy", SURF INT AN, 32(1), 2001, pp. 15-19

Abstract

In this work, we report observation of the fine structure of cobalt-hexammine-induced DNA condensates by tapping mode atomic force microscopy (AFM). A uniform system with minimum fluctuations of concentration was obtained byintroducing a slow-evaporation method, which enables investigation of the intermediate structures in DNA condensation. Some toroid condensates had well-defined small globule subunits; some other condensates were composed of nodular sections with gauze-like complexes spreading around at the outskirts; typical images revealed badly assembled toroidal condensates composed ofsmall globules with a fraction of loosely associated globules dotted in the gauze-like complexes spreading around the badly-assembled part of the toroid. These observations indicate that DNA condensation seems to involve five major stages: initial rapid association of chains to form gauze-like complexes; collapse of these gauze-like complexes at some critical sizes; growth of the critical nucleus by accretion, formation of rods by aggregation ofcondensed particles; and association of rod-like condensates to form toroids. The results may shed light on uncovering the physical mechanism of DNA condensation by multivalent cations. Copyright (C) 2001 John Wiley & Sons, Ltd.

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Documento generato il 09/07/20 alle ore 20:18:34