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Titolo:
Hypoxia combined with Escherichia coli produces irreversible gut mucosal injury characterized by increased intestinal cytokine production and DNA degradation
Autore:
Ding, J; Magnotti, LJ; Huang, QH; Xu, DZ; Condon, MR; Deitch, EA;
Indirizzi:
Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Surg, Newark, NJ 07103 USA Univ Med & Dent New Jersey Newark NJ USA 07103 Surg, Newark, NJ 07103 USA
Titolo Testata:
SHOCK
fascicolo: 3, volume: 16, anno: 2001,
pagine: 189 - 195
SICI:
1073-2322(200109)16:3<189:HCWECP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MULTIPLE-ORGAN-FAILURE; BACTERIAL TRANSLOCATION; HEMORRHAGIC-SHOCK; SELECTIVE DECONTAMINATION; MESENTERIC LYMPH; DIGESTIVE-TRACT; INTENSIVE-CARE; LUNG INJURY; PERMEABILITY;
Keywords:
ischemia/reperfusion; Ussing chamber; ileal membrane; bacterial translocation; gut associated lymphoid tissue;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Deitch, EA Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Surg, MSB G506,185 S Orange Ave, Newark, NJ 07103 USA Univ Med & Dent New Jersey MSB G506,185 S Orange Ave Newark NJ USA 07103
Citazione:
J. Ding et al., "Hypoxia combined with Escherichia coli produces irreversible gut mucosal injury characterized by increased intestinal cytokine production and DNA degradation", SHOCK, 16(3), 2001, pp. 189-195

Abstract

The objective of the present study was to determine whether hypoxia/reoxygenation in the absence or presence of intestinal bacteria would affect the integrity of the gut mucosal epithelium (as evidenced by histologic changes) and increase the local production of cytokines (interleukin 6 [IL-6] and tumor necrosis factor [TNF]). Rat ileal mucosal membranes were harvested and their electrophysiologic properties and barrier function were measured exvivo in the Ussing chamber system. Membranes were exposed to normoxia, normoxia + Escherichia coli, hypoxia for 40 min followed by normoxia, or hypoxia for 40 min + E. coli followed by normoxia for 3 h. IL-6 and TNF levels were measured using cytokine-dependent cellular assays. Morphological changes and the degree of DNA fragmentation were used as quantitative markers of gut mucosal injury. Mucosal integrity was maintained in the normoxia group. The addition of bacteria increased the IL-6 response and reduced mucosal integrity. During the hypoxic period, a transient decline in resistance (R) occurred and cytokine production was reduced. In the hypoxic ileal membranes not exposed to E. coli, reoxygenation reversed the change in R and increased IL-6 production. The combination of hypoxia/reoxygenation plus E coli bacterial challenge resulted in the greatest extent of gut mucosal injury and increase in TNF production. The results of this study support the hypothesis that the combination of increased intestinal bacterial levels superimposed on an ischemia/reperfusion injury increases the magnitude of gut mucosal injury and the production and subsequent release of proinflammatory cytokines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:57:57