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Titolo:
Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats
Autore:
Lund, RD; Adamson, P; Sauve, Y; Keegan, DJ; Girman, SV; Wang, SM; Winton, H; Kanuga, N; Kwan, ASL; Beauchene, L; Zerbib, A; Hetherington, L; Couraud, PO; Coffey, P; Greenwood, J;
Indirizzi:
Univ Coll London, Inst Ophthalmol, Div Cell Biol, London EC1V 9EL, EnglandUniv Coll London London England EC1V 9EL Biol, London EC1V 9EL, England Univ Coll London, Inst Ophthalmol, Dept Pathol, London EC1V 9EL, England Univ Coll London London England EC1V 9EL athol, London EC1V 9EL, England Univ Utah, Moran Eye Ctr, Salt Lake City, UT 84132 USA Univ Utah Salt Lake City UT USA 84132 e Ctr, Salt Lake City, UT 84132 USA Univ Sheffield, Dept Psychol, Sheffield S10 2TP, S Yorkshire, England UnivSheffield Sheffield S Yorkshire England S10 2TP S Yorkshire, England Neurotech SA, Genopole Ind, F-91000 Evry, France Neurotech SA Evry France F-91000 SA, Genopole Ind, F-91000 Evry, France
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 17, volume: 98, anno: 2001,
pagine: 9942 - 9947
SICI:
0027-8424(20010814)98:17<9942:STOGMH>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINAL-PIGMENT EPITHELIUM; RCS RAT; ROYAL-COLLEGE; MACULAR DEGENERATION; SURGEONS RATS; RPE; GRAFTS; RESCUE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Greenwood, J Univ Coll London, Inst Ophthalmol, Div Cell Biol, 11-43 Bath St, London EC1V 9EL, England Univ Coll London 11-43 Bath St London EnglandEC1V 9EL gland
Citazione:
R.D. Lund et al., "Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats", P NAS US, 98(17), 2001, pp. 9942-9947

Abstract

Royal College of Surgeons rats are genetically predisposed to undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have examined the efficacy of subretinal transplantation of two independent human RIPE cell lines each exhibiting genetic modifications that confer long-term stability in vitro. Thetwo cell lines, a spontaneously derived cell line (ARPE19) and an extensively characterized genetically engineered human RIPE cell line (h1RPE7), which expresses SV40 large T (tumor) antigen, were evaluated separately. Both lines result in a significant preservation of visual function as assessed by either behavioral or physiological techniques. This attenuation of visualloss correlates with photoreceptor survival and the presence of donor cells in the areas of rescued photoreceptors at 5 months postgrafting (6 monthsof age). These results demonstrate the potential of genetically modified human RPE cells for ultimate application in therapeutic transplantation strategies for retinal degenerative diseases caused by RIPE dysfunction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:46:17