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Titolo:
Activation of salicylic acid-induced protein kinase, a mitogen-activated protein kinase, induces multiple defense responses in tobacco
Autore:
Zhang, SQ; Liu, YD;
Indirizzi:
Univ Missouri, Dept Biochem, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Dept Biochem, Columbia, MO 65211 USA
Titolo Testata:
PLANT CELL
fascicolo: 8, volume: 13, anno: 2001,
pagine: 1877 - 1889
SICI:
1040-4651(200108)13:8<1877:AOSAPK>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT-DISEASE RESISTANCE; MAP KINASE; SIGNAL-TRANSDUCTION; CELL-DEATH; IN-VIVO; TRANSIENT ACTIVATION; FUNCTIONAL-ANALYSIS; PATHOGEN DEFENSE; ELICITOR-SIGNAL; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, SQ Univ Missouri, Dept Biochem, 117 Schweitzer Hall, Columbia, MO 65211 USA Univ Missouri 117 Schweitzer Hall Columbia MO USA 65211 5211 USA
Citazione:
S.Q. Zhang e Y.D. Liu, "Activation of salicylic acid-induced protein kinase, a mitogen-activated protein kinase, induces multiple defense responses in tobacco", PL CELL, 13(8), 2001, pp. 1877-1889

Abstract

The activation of mitogen-activated protein kinases (MAPKs) is one of the earliest responses in plants challenged by avirulent pathogens or cells treated with pathogen-derived elicitors. Expression of a constitutively activeMAPK kinase, NtMEK2(DD), in tobacco induces the expression of defense genes and hypersensitive response-like cell death, which are preceded by the activation of two endogenous MAPKs, salicylic acid-induced protein kinase (SIPK) and wounding-induced protein kinase (WIPK). However, the roles that SIPK and WIPK each play in the process are unknown. Here we report that SIPK alone is sufficient to activate these defense responses. In tobacco leaves transiently transformed with SIPK under the control of a steroid-inducible promoter, the induction of SIPK expression after the application of dexamethasone, a steroid, leads to an increase of SIPK activity. The increase of SIPK activity is dependent on the phosphorylation of newly synthesized SIPK by its endogenous upstream kinase. In contrast, the expression of WIPK underthe same conditions fails to increase its activity, even though the protein accumulates to a similar level. Studies using chimeras of SIPK and WIPK demonstrated that the C terminus of SIPK contains the molecular determinant for its activation, which is rather surprising because the N termini of SIPK and WIPK are more divergent. SIPK has been implicated previously in the regulation of both plant defense gene activation and hypersensitive response-like cell death based on evidence from pharmacological studies using kinase inhibitors. This gain-of-function study provided more direct evidence forits role in the signaling of multiple defense responses in tobacco.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 11:41:16