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Titolo:
Thermodynamic stability of base pairs, between 2-hydroxyadenine and incoming nucleotides as a determinant of nucleotide incorporation specificity during replication
Autore:
Kawakami, J; Kamiya, H; Yasuda, K; Fujiki, OY; Kasai, H; Sugimoto, N;
Indirizzi:
Konan Univ, Fac Sci & Engn, Dept Chem, Higashinada Ku, Kobe, Hyogo 6588501, Japan Konan Univ Kobe Hyogo Japan 6588501 hinada Ku, Kobe, Hyogo 6588501, Japan Konan Univ, High Technol Res Ctr, Higashinada Ku, Kobe, Hyogo 6588501, Japan Konan Univ Kobe Hyogo Japan 6588501 hinada Ku, Kobe, Hyogo 6588501, Japan Univ Occupat & Environm Hlth, Inst Ind Ecol Sci, Dept Environm Oncol, Kitakyushu, Fukuoka 8078555, Japan Univ Occupat & Environm Hlth Kitakyushu Fukuoka Japan 8078555 8555, Japan
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 16, volume: 29, anno: 2001,
pagine: 3289 - 3296
SICI:
0305-1048(20010815)29:16<3289:TSOBPB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-HA-RAS; DNA-POLYMERASES; ESCHERICHIA-COLI; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; SEQUENCE CONTEXTS; OPPOSITE 2-HYDROXYADENINE; CHAIN ORIENTATION; HOT-SPOTS; 2'-DEOXYISOGUANOSINE; ISOGUANINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Sugimoto, N Konan Univ, Fac Sci & Engn, Dept Chem, Higashinada Ku, 8-9-1 Okamoto, Kobe, Hyogo 6588501, Japan Konan Univ 8-9-1 Okamoto Kobe Hyogo Japan 6588501 88501, Japan
Citazione:
J. Kawakami et al., "Thermodynamic stability of base pairs, between 2-hydroxyadenine and incoming nucleotides as a determinant of nucleotide incorporation specificity during replication", NUCL ACID R, 29(16), 2001, pp. 3289-3296

Abstract

We investigated the thermodynamic stability of double-stranded DNAs with an oxidative DNA lesion, 2-hydroxyadenine (2-OH-Ade), in two different sequence contexts (5'-GA*C-3' and 5'-TA*A-3, A* represents 2-OH-Ade). When an A*-N pair (N, any nucleotide base) was located in the center of a duplex, thethermodynamic stabilities of the duplexes were similar for all the naturalbases except A (N = T, C and G). On the other hand, for the duplexes with the A*-N pair at the end, which mimic the nucleotide incorporation step, the stabilities of the duplexes were dependent on their sequence. The order of stability is T > G > C >> A in the 5'-GA*C-3' sequences and T > A > C > Gin the 5'-TA*A-3' sequences. Because T/G/C and T/A are nucleotides, incorporated opposite to 2-OH-Ade in the 5-GA*C-3' and 5'-A*A-3' sequences, respectively, these results agree with the tendency of mutagenic misincorporation of the nucleotides opposite to 2-OH-Ade in vitro. Thus, the thermodynamicstability of the A*-N base pair may be an important factor for the mutation spectra of 2-OH-Ade.

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Documento generato il 09/07/20 alle ore 22:59:18