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Titolo:
First discrete autoradiographic distribution of aminopeptidase N in various structures of rat brain and spinal cord using the selective iodinated inhibitor [I-125]RB 129
Autore:
Noble, F; Banisadr, G; Jardinaud, F; Popovici, T; Lai-Kuen, R; Chen, H; Bischoff, L; Parsadaniantz, SM; Fournie-Zaluski, MC; Roques, BP;
Indirizzi:
UFR Sci Pharmaceut & Biol, INSERM, Dept Pharmacochim Mol & Struct, CNRS,U266, F-75270 Paris 06, France UFR Sci Pharmaceut & Biol Paris France 06 U266, F-75270 Paris 06, France Hop St Antoine, INSERM, U339, F-75571 Paris, France Hop St Antoine ParisFrance F-75571 INSERM, U339, F-75571 Paris, France UFR Sci Pharmaceut & Biol, Dept Microscopie Elect, F-75270 Paris 06, France UFR Sci Pharmaceut & Biol Paris France 06 lect, F-75270 Paris 06, France
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 105, anno: 2001,
pagine: 479 - 488
SICI:
0306-4522(2001)105:2<479:FDADOA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANGIOTENSIN-CONVERTING ENZYME; ENKEPHALIN-DEGRADING ENZYMES; NEUTRAL ENDOPEPTIDASE; NOCICEPTIN/ORPHANIN FQ; REGIONAL DISTRIBUTION; HIGHLY POTENT; OPIOID RECEPTORS; HPA AXIS; LOCALIZATION; METABOLISM;
Keywords:
enkephalins; neutral endopeptidase; intestine; aminopeptidase N inhibitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Roques, BP UFR Sci Pharmaceut & Biol, INSERM, Dept Pharmacochim Mol & Struct, CNRS,U266, 4 Ave Observ, F-75270 Paris 06, France UFR Sci Pharmaceut & Biol 4 Ave Observ Paris France 06 France
Citazione:
F. Noble et al., "First discrete autoradiographic distribution of aminopeptidase N in various structures of rat brain and spinal cord using the selective iodinated inhibitor [I-125]RB 129", NEUROSCIENC, 105(2), 2001, pp. 479-488

Abstract

The selective and potent aminopeptidase N inhibitor [I-125]RB 129 has beenused for the radioantographic localization of this enzyme in rat brain, spinal cord and intestine. Brain microvessels and intestine brush-border cells were shown to present a high concentration of aminopeptidase N. Moreover,a labeling of various brain structures was observed. A very high level of binding occurred in the meninges, choroid plexus, pineal gland, paraventricular nucleus and pituitary gland. Moderate to high labeling was also observed in the cortex, caudate-putamen, subthalamic nucleus, central periaqueductal gray, thalamus, as well as in the dorsal and ventral horn of the spinalcord, which are known to contain a high concentration of enkephalins, opioid receptors and neutral endopeptidase. This co-localization confirms the physiological implication of aminopeptidase N in the inactivation of enkephalins accounting for the requirement of dual inhibition of neutral endopeptidase and aminopeptidase N to observe highly significant morphine-like effects induced by the protected endogenous opioid peptides. Aminopeptidase N was also visualized in moderate to high levels in other brain structures suchas the hippocampus, nucleus accumbens, substantia nigra, hypothalamus (dorsomedial and ventromedial nuclei), raphe nucleus, pontine nucleus, inferiorolive, and in high concentration in the granular layer of cerebellum. In summary, aminopoptidase N has been visualized for the first time in numerous brain areas using the selective inhibitor [I-125]RB 129. This iodinated probe could allow the ey vivo and in vivo localization of aminopeptidaseN in various tissues to be investigated and may also be used to evaluate quantitative changes in aminopeptidase N expression in pathological situations. Aminopeptidase N, which preferably removes NH2-terminal neutral amino acids from peptides, has probably a host of substrates, Nevertheless, a certain in vivo selectivity could be achieved by the presence of the enzyme in structures where the peptide effector and its receptors are also co-localized. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:32:39