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Titolo:
The development of inhibitory synaptic specializations in the mouse deep cerebellar nuclei
Autore:
Garin, N; Escher, G;
Indirizzi:
Inst Biol Cellulaire & Morphol, CH-1005 Lausanne, Switzerland Inst Biol Cellulaire & Morphol Lausanne Switzerland CH-1005 Switzerland
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 105, anno: 2001,
pagine: 431 - 441
SICI:
0306-4522(2001)105:2<431:TDOISS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBUNIT-SPECIFIC ANTIBODIES; RAT SPINAL-CORD; GABA(A) RECEPTORS; OLFACTORY-BULB; MUTANT MICE; GABA(A)-RECEPTOR SUBTYPES; BENZODIAZEPINE RECEPTOR; POSTNATAL-DEVELOPMENT; PURKINJE-CELLS; MESSENGER-RNA;
Keywords:
GABA(A); cerebellum; synaptogenesis; aggregation; morphology; confocal;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Garin, N Inst Biol Cellulaire & Morphol, Bugnon 9, CH-1005 Lausanne, Switzerland Inst Biol Cellulaire & Morphol Bugnon 9 Lausanne Switzerland CH-1005
Citazione:
N. Garin e G. Escher, "The development of inhibitory synaptic specializations in the mouse deep cerebellar nuclei", NEUROSCIENC, 105(2), 2001, pp. 431-441

Abstract

Using confocal laser scanning microscopy and immunohistochemistry, this study shows the complete morphological development of GABAergic synaptic contacts between Purkinje cells and neurons of the deep cerebellar nuclei of the mouse. Firstly, presynaptic varicosities visualized with antibodies against synaptophysin, synapsin or glutamic acid decarboxylase, were detected when the postsynaptic GABAA receptors were not yet aggregated in the membranebut had a diffuse cytoplasmic distribution, which indicated a lead in maturation of presynaptic terminals over target cells. Secondly, receptor aggregates developed suddenly after an initial week of diffuse expression and these clusters matured into more numerous and larger synaptic aggregates. During this postsynaptic maturation, the presynaptic varicosities develop intonumerous and well-defined spots. As soon as both pre- and postsynaptic clusters were detectable, these sites are always colocalized. We therefore consider the aggregation of postsynaptic; receptor during development as a landmark of synapse formation. Our observations are consistent with a developmental model in which the presynaptic neuron differentiates its axon before the target neuron expressesthe mature form of its receptors on the membrane. The presynaptic neuron can therefore instruct the target neuron about the distribution and aggregation of the postsynaptic receptors at the synapse. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:25:33