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Titolo:
The role of the globus pallidus D2 subfamily of dopamine receptors in pallidal immediate early gene expression
Autore:
Marshall, JF; Henry, BL; Billings, LM; Hoover, BR;
Indirizzi:
Univ Calif Irvine, Dept Neurobiol & Behav, Irvine, CA 92697 USA Univ CalifIrvine Irvine CA USA 92697 obiol & Behav, Irvine, CA 92697 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 105, anno: 2001,
pagine: 365 - 378
SICI:
0306-4522(2001)105:2<365:TROTGP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-FOS GENE; PARVALBUMIN-IMMUNOREACTIVE NEURONS; CALCIUM-BINDING PROTEINS; RAT STRIATAL NEURONS; FREELY MOVING RATS; MESSENGER-RNA; STRIATOPALLIDAL NEURONS; SUBSTANTIA-NIGRA; BASAL GANGLIA; N-ETHOXYCARBONYL-2-ETHOXY-1,2-DIHYDROQUINOLINE INVIVO;
Keywords:
basal ganglia; N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline; Fos; striatum; substituted benzamide; transcription factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
94
Recensione:
Indirizzi per estratti:
Indirizzo: Marshall, JF Univ Calif Irvine, Dept Neurobiol & Behav, 2215 Bio Sci 2, Irvine, CA 92697 USA Univ Calif Irvine 2215 Bio Sci 2 Irvine CA USA 92697 2697 USA
Citazione:
J.F. Marshall et al., "The role of the globus pallidus D2 subfamily of dopamine receptors in pallidal immediate early gene expression", NEUROSCIENC, 105(2), 2001, pp. 365-378

Abstract

The globus pallidus plays an important role in basal ganglia circuitry, representing the first relay nucleus of the 'indirect pathway' of striatal efferents. In contrast to the well-characterized actions of dopamine on striatal neurons, the functional role of the dopamine innervation of globus pallidus is less well understood. Previous research showed that systemic administration of either a dopamine D2 receptor antagonist or combined dopamine DI and D2 receptor agonists induces Fos, the protein product of the immediate early gene c-fos, in neurons of globus pallidus [Ruskin and Marshall (1997) Neuroscience 81, 79-92]. To determine whether the ability of the D2 receptor antagonist, sulpiride, to induce Fos in rat pallidal neurons is mediated by D2-like receptors in striatum or globus pallidus, intrastriatal or intrapallidal sulpiride infusions were conducted. The diffusion of intrastriatal sulpiride was estimated by measuring this antagonist's competition for N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ)-induced D2 receptor inactivation. The phenotype of the striatal neurons expressing Fos after intrastriatal infusion was assessed by combining Fos immunocytochemistry with D2 receptor mRNA in situ hybridization. Intrastriatal infusions of (-)-sulpiride (10-200 ng) dose-dependently increased the number of striatal cells expressing Fos; and the Fos-immunoreactive striatal cells were D2 receptor mRNA-expressing, the same population in which systemic D2 receptor antagonists induce Fos. Intrastriatal infusions of high (5 mug), but not low (10-200ng), (-)-sulpiride doses also induced Fos in globus pallidus cells but thesulpiride appeared to spread to the globus pallidus. Direct intrapallidal infusions of (-)-sulpiride (50-100 ng) dose-dependently induced Fos in globus pallidus with minimal influence on striatum or other basal ganglia structures. Using sensitive in situ hybridization conditions, prominent labelingof D2 receptor mRNA was evident in globus pallidus. D2 receptor mRNA was densest in a lateral 200 mum wide band that follows the curvature of the pallidal/striatal boundary. Cellular analysis revealed silver clusters associated with D2 receptor mRNA labeling over globus pallidus neurons that were immunoreactive for neuron-specific nuclear protein. These results strongly suggest that the dopaminergic innervation of globuspallidus, acting through D2-like receptors internal to this structure, cancontrol gene expression in pallidal neurons. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 00:47:42