Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Up-regulation of secretoneurin immunoreactivity and secretogranin II mRNA in rat striatum following 6-hydroxydopamine lesioning and chronic L-dopa treatment
Autore:
Medhurst, AD; Zeng, BY; Charles, KJ; Gray, J; Reavill, C; Hunter, AJ; Shale, JA; Jenner, P;
Indirizzi:
GlaxoSmithKline, Neurosci Res, Harlow CM19 5AW, Essex, England GlaxoSmithKline Harlow Essex England CM19 5AW ow CM19 5AW, Essex, England Univ London Kings Coll, GKT Sch Biomed Sci, Neurodegenerat Dis Res Ctr, London SE1 1UL, England Univ London Kings Coll London England SE1 1UL r, London SE1 1UL, England
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 105, anno: 2001,
pagine: 353 - 364
SICI:
0306-4522(2001)105:2<353:UOSIAS>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR MESSENGER-RNA; GENE-EXPRESSION; PARKINSONS-DISEASE; NIGROSTRIATAL PATHWAY; DIFFERENTIAL DISPLAY; CAUDATE-PUTAMEN; CHROMOGRANIN-C; BASAL GANGLIA; SUBSTANCE-P; BRAIN;
Keywords:
nigral-striatal pathway; differential display; up-regulation; TaqMan; gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Medhurst, AD GlaxoSmithKline, Neurosci Res, Harlow CM19 5AW, Essex, England GlaxoSmithKline Harlow Essex England CM19 5AW Essex, England
Citazione:
A.D. Medhurst et al., "Up-regulation of secretoneurin immunoreactivity and secretogranin II mRNA in rat striatum following 6-hydroxydopamine lesioning and chronic L-dopa treatment", NEUROSCIENC, 105(2), 2001, pp. 353-364

Abstract

Destruction of the nigro-striatal pathway in Parkinson's disease and treatment with L-DOPA lead to persistent alterations in basal ganglia output pathways that are poorly characterised. Differential display mRNA analysis wasused to study the effects of 6-hydroxydopamine-induced lesions of the medial forebrain bundle on gene expression in the rat striatum. One up-regulated cDNA identified in two independent groups of 6-hydroxydopamine-lesioned animals was cloned and sequence analysis showed 97% homology to secretogranin IT. Differential up-regulation of secretogranin IT following 6-hydroxydopamine lesioning was confirmed in a further group of 6-hydroxydopamine-lesioned rats using TaqMan real time quantitative reverse transcript ion-polymerase chain reaction. Following chronic L-DOPA treatment of 6-hydroxydopamine-lesioned rats, secretogranin II mRNA was further up-regulated to a similardegree to that observed for preproenkephalin A mRNA expression. Immunohistochemical analysis confirmed the increase in secretogranin IT peptide levels in striatal neurotics in 6-hydroxydopamine-lesioned rats following chronic L-DOPA treatment,The increase in secretogranin II mRNA occurring following destruction of the nigro-striatal pathway and chronic L-DOPA treatment may result in an increase in secretoneurin levels, which could be important for the regulation of striatal output pathways. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 10:18:14