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Titolo:
Intracellular generation of free radicals and modifications of detoxifyingenzymes in cultured neurons from the developing rat forebrain in response to transient hypoxia
Autore:
Lievre, V; Becuwe, P; Bianchi, A; Bossenmeyer-Pourie, C; Koziel, V; Franck, P; Nicolas, MB; Dauca, M; Vert, P; Daval, JL;
Indirizzi:
Univ Henri Poincare, JE 2164, Nancy, France Univ Henri Poincare Nancy France Henri Poincare, JE 2164, Nancy, France Univ Henri Poincare, EA 2402, Nancy, France Univ Henri Poincare Nancy France Henri Poincare, EA 2402, Nancy, France
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 105, anno: 2001,
pagine: 287 - 297
SICI:
0306-4522(2001)105:2<287:IGOFRA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
MANGANESE-SUPEROXIDE-DISMUTASE; GUINEA-PIG BRAIN; OXYGEN-FREE RADICALS; SOD MESSENGER-RNA; GLUTATHIONE-PEROXIDASE; CELL-DEATH; LIPID-PEROXIDATION; INDUCED APOPTOSIS; OXIDATIVE STRESS; ISCHEMIC-INJURY;
Keywords:
immature brain; hypoxia/reoxygenation; apoptosis; oxidative stress; endogenous defense system;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
84
Recensione:
Indirizzi per estratti:
Indirizzo: Daval, JL INSERM, Fac Med, EMI 0014, 9 Ave Foret Haye,POB 184, F-54505 Vandoeuvre Les Nancy, France INSERM 9 Ave Foret Haye,POB 184 Vandoeuvre Les Nancy France F-54505
Citazione:
V. Lievre et al., "Intracellular generation of free radicals and modifications of detoxifyingenzymes in cultured neurons from the developing rat forebrain in response to transient hypoxia", NEUROSCIENC, 105(2), 2001, pp. 287-297

Abstract

To address the influence of oxidative stress and defense capacities in theeffects of transient hypoxia in the immature brain, the time course of reactive oxygen species generation was monitored by flow cytometry using dihydrorhodamine 123 and 2',7'-dichlorofluorescein-diacetate in cultured neuronsissued from the fetal rat forebrain and subjected to hypoxia/reoxygenation(6 h/96 h). Parallel transcriptional and activity changes of superoxide dismutases, glutathione peroxidase and catalase were analyzed, in line with cell outcome. The study confirmed hypoxia-induced delayed apoptotic death, and depicted increased mitochondrial and cytosolic productions of free radicals (+30%) occurring over the 48-h period after the restoration of oxygen supply, with sequential stimulations of superoxide dismutases. Whereas catalase mRNA levels and activity were augmented by cell reoxygenation, glutathione peroxidase activity was transiently repressed (-24%), along with reduced glutathione reductase activity (-27%) and intracellular glutathione depletion (-19%). Coupled with the neuroprotective effects of the glutathione precursor N-acetyl-cysteine (50 muM), these data suggest that hypoxia/reoxygenation-induced production of reactive oxygen species can overwhelm glutathione-dependent antioxidant capacity, and thus may contribute to the resulting neuronal apoptosis. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 19:15:32