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Titolo:
Telomere lengths and telomerase activity in dog tissues: A potential modelsystem to study human telomere and telomerase biology
Autore:
Nasir, L; Devlin, P; Mckevitt, T; Rutteman, G; Argyle, DJ;
Indirizzi:
Univ Glasgow, Sch Vet, Dept Vet Clin Studies, Glasgow G61 1QH, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G61 1QH ow G61 1QH, Lanark, Scotland Waltham Ctr Pet Nutr, Melton Mobray, Leics, England Waltham Ctr Pet Nutr Melton Mobray Leics England Mobray, Leics, England Univ Utrecht, Fac Vet Med, Dept Clin Sci Compan Anim, NL-3508 TD Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3508 TD 3508 TD Utrecht, Netherlands
Titolo Testata:
NEOPLASIA
fascicolo: 4, volume: 3, anno: 2001,
pagine: 351 - 359
SICI:
1522-8002(200107/08)3:4<351:TLATAI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC-CELLS; HUMAN FIBROBLASTS; HUMAN-LYMPHOCYTES; MOUSE TELOMERASE; IMMORTAL CELLS; BLOOD-CELLS; CANCER; MICE; DNA; CARCINOMA;
Keywords:
telomere; telomerase; dog; cancer; model;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Nasir, L Univ Glasgow, Sch Vet, Dept Vet Clin Studies, Bearsden Rd, Glasgow G61 1QH, Lanark, Scotland Univ Glasgow Bearsden Rd Glasgow Lanark Scotland G61 1QH Scotland
Citazione:
L. Nasir et al., "Telomere lengths and telomerase activity in dog tissues: A potential modelsystem to study human telomere and telomerase biology", NEOPLASIA, 3(4), 2001, pp. 351-359

Abstract

Studies on telomere and telomerase biology are fundamental to the understanding of aging and age-related diseases such as cancer. However, human studies have been hindered by differences in telomere biology between humans and the classical murine animal model system. In this paper, we describe basic studies of telomere length and telomerase activity in canine normal and neoplastic tissues and propose the dog as an alternative model system. Briefly, telomere lengths were measured in normal canine peripheral blood mononuclear cells (PBMCs), a range of normal canine tissues, and in a panel of naturally occurring soft tissue tumours by terminal restriction fragment (TRF) analysis. Further, telomerase activity was measured in canine cell lines and multiple canine tissues using a combined polymerase chain reaction/enzyme-linked immunosorbent assay method. TRF analysis in canine PBMCs and tissues demonstrated mean TRF lengths to range between 12 and 23 kbp with heterogeneity in telomere lengths being observed in a range of normal somatic tissues. In soft tissue sarcomas, two subgroups were identified with mean TRFs of 22.2 and 18.2 kbp. Telomerase activity in canine tissue was present intumour tissue and testis with little or no activity in normal somatic tissues. These results suggest that the dog telomere biology is similar to thatin humans and may represent an alternative model system for studying telomere biology and telomerase-targeted anticancer therapies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 20:09:38