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Titolo:
Downregulation of Akt1 inhibits anchorage-independent cell growth and induces apoptosis in cancer cells
Autore:
Liu, XS; Shi, Y; Han, EKH; Chen, ZH; Rosenberg, SH; Giranda, VL; Luo, Y; Ng, SC;
Indirizzi:
Abbott Labs, Dept 47S, Div Pharmaceut Prod, Abbott Pk, IL 60064 USA AbbottLabs Abbott Pk IL USA 60064 armaceut Prod, Abbott Pk, IL 60064 USA
Titolo Testata:
NEOPLASIA
fascicolo: 4, volume: 3, anno: 2001,
pagine: 278 - 286
SICI:
1522-8002(200107/08)3:4<278:DOAIAC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-B; PLECKSTRIN HOMOLOGY DOMAIN; MOLECULAR-CLONING; PHOSPHATIDYLINOSITOL 3-KINASE; PROSTATE-CANCER; COMBINATION CHEMOTHERAPY; CYTOCHROME-C; ACTIVATION; ONCOGENE; SURVIVAL;
Keywords:
Akt1; apoptosis; antisense; oligonucleotide; cancer; combination treatment;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, XS Abbott Labs, Dept 47S, Div Pharmaceut Prod, AP9,100 Abbott Pk Rd, Abbott Pk, IL 60064 USA Abbott Labs AP9,100 Abbott Pk Rd Abbott Pk IL USA 60064 60064 USA
Citazione:
X.S. Liu et al., "Downregulation of Akt1 inhibits anchorage-independent cell growth and induces apoptosis in cancer cells", NEOPLASIA, 3(4), 2001, pp. 278-286

Abstract

The serine/threonine kinases, Akt1/PKB alpha, Akt2/PKB beta, and Akt3/PKB-gamma, play a critical role in preventing cancer cells from undergoing apoptosis. However, the function of individual Akt isoforms in the tumorigenicity of cancer cells is still not well defined. In the current study, we usedan Akt1 antisense oligonucleotide (AS) to specifically downregulate Akt1 protein in both cancer and normal cells. Our data indicate that Akt1 AS treatment inhibits the ability of MiaPaCa-2, H460, HCT-15, and HT1080 cells to grow in soft agar. The treatment also induces apoptosis in these cancer cells as demonstrated by FACS analysis and a caspase activity assay. Conversely, Akt1 AS treatment has little effect on the cell growth and survival of normal human cells including normal human fibroblast (NHF), fibroblast from muscle (FBM), and mammary gland epithelial 184B5 cells. In addition, Akt1 AS specifically sensitizes cancer cells to typical chemotherapeutic agents. Thus, Akt1 is indispensable for maintaining the tumorigenicity of cancer cells. Inhibition of Akt1 may provide a powerful sensitization agent for chemotherapy specifically in cancer cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/02/20 alle ore 08:15:13