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Titolo:
Induction of a hypersensitive response by chimeric helicase sequences of tobamoviruses U1 and Ob in N-carrying tobacco
Autore:
Abbink, TEM; de Vogel, J; Bol, JF; Linthorst, HJM;
Indirizzi:
Leiden Univ, Gorlaeus Labs, Inst Mol Plant Sci, NL-2300 RA Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RA NL-2300 RA Leiden, Netherlands
Titolo Testata:
MOLECULAR PLANT-MICROBE INTERACTIONS
fascicolo: 9, volume: 14, anno: 2001,
pagine: 1086 - 1095
SICI:
0894-0282(200109)14:9<1086:IOAHRB>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT-DISEASE-RESISTANCE; SYSTEMIC ACQUIRED-RESISTANCE; LEUCINE-RICH REPEATS; ELICITOR-ACTIVE SITE; FOR-GENE CONCEPT; COAT PROTEIN; IDENTIFICATION; PATHWAYS; PRODUCT; DOMAIN;
Keywords:
avirulence; gene for gene; protein-protein interaction; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Linthorst, HJM Leiden Univ, Gorlaeus Labs, Inst Mol Plant Sci, POB 9502, NL-2300 RA Leiden, Netherlands Leiden Univ POB 9502 Leiden Netherlands NL-2300 RA erlands
Citazione:
T.E.M. Abbink et al., "Induction of a hypersensitive response by chimeric helicase sequences of tobamoviruses U1 and Ob in N-carrying tobacco", MOL PL MICR, 14(9), 2001, pp. 1086-1095

Abstract

Recently, the helicase domain of the Tobacco mosaic virus (TMV)-U1 replicase proteins (designated MOREHEL:U1) was identified as the elicitor of the Ngene-mediated hypersensitive response (HR) in tobacco. In this study, we used agroinfiltration to express the equivalent MOREHEL domain of the non-HR-inducing tobamovirus strain TMV-Ob. It appeared that this MOREHEL:Ob sequence did not elicit a HR in N gene-carrying tobacco. Both MOREHEL sequences were divided into eight subdomains, and chimeras of MOREHEL sequences from U1 and Ob were constructed. Expression of these chimeric MOREHEL sequences revealed that, in the TMV-U1 MOREHEL sequence, at least four domains involved in full HR induction were present. The presence of at least three of these four domains seems a minimal requirement for HR induction. Two additional domains may play a minor role in HR induction. To study the elicitor function of the chimeras during the TMV life cycle, chimeric MOREHEL domains were introduced into full-length TMV cDNA clones. These constructs, however, were unable to establish an infection in Nicotiana benthamiana or Nicotianatabacum plants.

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Documento generato il 31/03/20 alle ore 08:36:34