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Titolo:
Functional expression of Cf9 and and Avr9 genes in Brassica napus induces enhanced resistance to Leptosphaeria maculans
Autore:
Hennin, C; Hofte, M; Diederichsen, E;
Indirizzi:
Aventis CropSci, B-9000 Ghent, Belgium Aventis CropSci Ghent Belgium B-9000 ntis CropSci, B-9000 Ghent, Belgium State Univ Ghent, Fac Agr & Appl Biol Sci, Phytopathol Lab, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 opathol Lab, B-9000 Ghent, Belgium
Titolo Testata:
MOLECULAR PLANT-MICROBE INTERACTIONS
fascicolo: 9, volume: 14, anno: 2001,
pagine: 1075 - 1085
SICI:
0894-0282(200109)14:9<1075:FEOCAA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGEN CLADOSPORIUM-FULVUM; RACE-SPECIFIC ELICITORS; HYPERSENSITIVE RESPONSE; DISEASE-RESISTANCE; CELL-DEATH; SALICYLIC-ACID; ACQUIRED-RESISTANCE; TRANSGENIC TOBACCO; DEFENSE RESPONSES; TOMATO PLANTS;
Keywords:
defense responses; gene-for-gene concept; transgenic plants; two-component system;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Diederichsen, E Aventis CropSci, J Plateaustr 22, B-9000 Ghent, Belgium Aventis CropSci J Plateaustr 22 Ghent Belgium B-9000 gium
Citazione:
C. Hennin et al., "Functional expression of Cf9 and and Avr9 genes in Brassica napus induces enhanced resistance to Leptosphaeria maculans", MOL PL MICR, 14(9), 2001, pp. 1075-1085

Abstract

The tomato Cf9 resistance gene induces an Avr9-dependent hypersensitive response (HR) in tomato and transgenic Solanaceae spp. We studied whether theCf9 gene product responded functionally to the corresponding Avr9 gene product when introduced in a heterologous plant species. We successfully expressed the Cf9 gene under control of its own promoter and the Avr9 or Avr9R8Kgenes under control of the p35S(1) promoter in transgenic oilseed rape. Wedemonstrated that the transgenic oilseed rape plants produced the Avr9 elicitor with the same specific necrosis-inducing activity as reported for Cladosporium fulvum. An Avr9-dependent HR was induced in Cf9 oilseed rape uponinjection of intercellular fluid containing Avr9. We showed Avr9-specific induction of PR1, PR2, and Cxc750 defense genes in oilseed rape expressing Cf9. Cf9 x Avr9 oilseed rape did not result in seedling death of the F-1 progeny, independent of the promoters used to express the genes. The F-1 (Cf9x Avr9) plants, however, were quantitatively more resistant to Leptosphaeria maculans. Phytopathological analyses revealed that disease development of L. maculans was delayed when the pathogen was applied on an Avr9-mediatedHR site. We demonstrate that the Cf9 and Avr9 gene can be functionally expressed in a heterologous plant species and that the two components confer an increase in disease resistance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:45:32