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Titolo:
Identification of the Helicobacter pylori anti-sigma(28) factor
Autore:
Colland, F; Rain, JC; Gounon, P; Labigne, A; Legrain, P; De Reuse, H;
Indirizzi:
Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France Inst Pasteur Paris France 15 erienne Muqueuses, F-75724 Paris 15, France Hybrigenics SA, F-75012 Paris, France Hybrigenics SA Paris France F-75012 ybrigenics SA, F-75012 Paris, France Inst Pasteur, Stn Cent Microscopie Elect, F-75724 Paris 15, France Inst Pasteur Paris France 15 Microscopie Elect, F-75724 Paris 15, France
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 2, volume: 41, anno: 2001,
pagine: 477 - 487
SICI:
0950-382X(200107)41:2<477:IOTHPA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTI-SIGMA FACTOR; FLAGELLUM-SPECIFIC SIGMA; COMPLETE GENOME SEQUENCE; SALMONELLA-TYPHIMURIUM; TRANSCRIPTIONAL REGULATION; CAMPYLOBACTER-JEJUNI; GENETIC-CHARACTERIZATION; ESCHERICHIA-COLI; NEGATIVE MUTANTS; ALLELIC EXCHANGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: De Reuse, H Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, 28 Rue Dr Roux, F-75724 Paris 15, France Inst Pasteur 28 Rue Dr Roux Paris France 15 Paris 15, France
Citazione:
F. Colland et al., "Identification of the Helicobacter pylori anti-sigma(28) factor", MOL MICROB, 41(2), 2001, pp. 477-487

Abstract

Flagellar motility is essential for colonization of the human gastric mucosa by Helicobacter pylori. The flagellar filament is composed of two subunits, FlaA and FlaB. Transcription of the genes encoding these proteins is controlled by the sigma (28) and sigma (54) factors of RNA polymerase respectively. The expression of flagellar genes is regulated, but no sigma (28)-specific effector was identified. It was also unclear whether H. pylori possessed a checkpoint for flagellar synthesis, and no gene encoding an anti-sigma (28) factor, FIgM, could be identified by sequence similarity searches. To investigate the sigma (28)-dependent regulation, a new approach based ongenomic data was used. Two-hybrid screening with the H. pylori proteins identified a protein of unknown function (HP1122 interacting with the sigma (28) factor and defined the C-terminal part of HP1122 (residues 48-76) as the interaction domain. HP1122 interacts with region 4 of sigma (28) and prevents its association with the beta -region of H. pylori RNA polymerase. Thus, HP1122 presented the characteristics of an anti-sigma (28) factor. This was confirmed in H. pylori by RNA dot-blot hybridization and electron microscopy. The level of sigma (28)-dependent flaA transcription was higher in aHP1122-deficient strain and was decreased by the overproduction of HP1122. The overproduction of HP1122 also resulted in H. pylori cells with highly truncated flagella. These results demonstrate that HP1122 is the H. pylori anti-sigma (28) factor, FIgM, a major regulator of flagellum assembly. Potential anti-sigma (28) factors were identified in Campylobacter jejuni, Pseudomonas aeruginosa and Thermotoga maritima by sequence homology with the C-terminal region of HP1122.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:10:03