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Titolo:
Purification and characterization of insulin-mimetic inositol phosphoglycan-like molecules from grass pea (Lathyrus sativus) seeds
Autore:
Paneda, C; Villar, AV; Alonso, A; Goni, FM; Varela, F; Brodbeck, U; Leon, Y; Varela-Nieto, I; Jones, DR;
Indirizzi:
CSIC, Inst Invest Biomed Alberto Sols, Madrid 28029, Spain CSIC Madrid Spain 28029 Invest Biomed Alberto Sols, Madrid 28029, Spain Univ Pais Vasco, Unidad Biofis, CSIC, EHU, E-48080 Bilbao, Spain Univ PaisVasco Bilbao Spain E-48080 s, CSIC, EHU, E-48080 Bilbao, Spain Univ Pais Vasco, Dept Bioquim, E-48080 Bilbao, Spain Univ Pais Vasco Bilbao Spain E-48080 Dept Bioquim, E-48080 Bilbao, Spain INIA, CRF, Madrid, Spain INIA Madrid SpainINIA, CRF, Madrid, Spain Univ Bern, Inst Biochem & Mol Biol, Bern, Switzerland Univ Bern Bern Switzerland , Inst Biochem & Mol Biol, Bern, Switzerland CSIC, Ctr Nacl Biotecnol, Dept Immunol & Oncol, Madrid, Spain CSIC Madrid Spain r Nacl Biotecnol, Dept Immunol & Oncol, Madrid, Spain
Titolo Testata:
MOLECULAR MEDICINE
fascicolo: 7, volume: 7, anno: 2001,
pagine: 454 - 460
SICI:
1076-1551(200107)7:7<454:PACOII>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
STREPTOZOTOCIN-DIABETIC RATS; BILOBA EXTRACT EGB-761; CELL NUCLEAR ANTIGEN; GROWTH-FACTOR-I; GLYCOSYL-PHOSPHATIDYLINOSITOL; INOSITOLPHOSPHOGLYCAN MEDIATORS; LIPOSOMAL MEMBRANES; BIOLOGICAL-ACTIVITY; PLANT TREATMENTS; INNER-EAR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Varela-Nieto, I CSIC, Inst Invest Biomed Alberto Sols, Arturo Duperier 4, Madrid 28029, Spain CSIC Arturo Duperier 4 Madrid Spain 28029 id 28029, Spain
Citazione:
C. Paneda et al., "Purification and characterization of insulin-mimetic inositol phosphoglycan-like molecules from grass pea (Lathyrus sativus) seeds", MOL MED, 7(7), 2001, pp. 454-460

Abstract

Background: Signal transduction through the hydrolysis of glycosyl-phosphatidylinositol (GPI) leading to the release of the water-soluble inositol phosphoglycan (IPG) molecules has been demonstrated to be important for mediating some of the actions of insulin and insulin-like growth factor-I (IGF-I). Materials and Methods: in the present study, GPI from grass pea (Lathyrus sativus) seeds has been purified and partially characterized on the basis of its chromatographic properties and its compositional analysis. Results: The results indicate that it shows similarities to GPI previouslyisolated from other sources such as rat liver. IPG was generated from L. sativus seed GPI by hydrolysis with a GPI-specific phospholipase D (GPI-PLD). This IPG inhibited protein kinase A (PKA) in an in vitro assay, caused cell proliferation in explanted cochleovestibular ganglia (CVG), and decreased 8-Br-cAMP-induced phosphoenolpyruvate carboxykinase (PEPCK) mRNA expression in cultured hepatoma cells. Conclusions: Our data indicate that L. sativus seed IPG possess insulin-mimetic activities. This may explain why L. sativus seeds have been used in some traditional medicines to ameliorate diabetic symptoms.

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Documento generato il 09/04/20 alle ore 06:18:18