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Titolo:
Molecular characterization of a deletion/duplication rearrangement in tfd genes from Ralstonia eutropha JMP134(pJP4) that improves growth on 3-chlorobenzoic acid but abolishes growth on 2,4-dichlorophenoxyacetic acid
Autore:
Clement, P; Pieper, DH; Gonzalez, B;
Indirizzi:
Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Mol Genet & Microbiol, Microbiol Lab, Santiago, Chile Pontificia Univ Catolica Chile Santiago Chile biol Lab, Santiago, Chile Natl Res Ctr Biotechnol, GBF, Div Microbiol, Braunschweig, Germany Natl Res Ctr Biotechnol Braunschweig Germany iol, Braunschweig, Germany
Titolo Testata:
MICROBIOLOGY-SGM
, volume: 147, anno: 2001,
parte:, 8
pagine: 2141 - 2148
SICI:
1350-0872(200108)147:<2141:MCOADR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
INCOMPATIBILITY GROUP-P; ALCALIGENES-EUTROPHUS; PLASMID PJP4; HERBICIDE 2,4-DICHLOROPHENOXYACETATE; CHLOROCATECHOL DEGRADATION; METABOLISM; INSERTION; ELEMENT; STRAIN; ORGANIZATION;
Keywords:
chloroaromatics; catabolic plasmid; IS1071 insertion sequence; chlorocatechol pathway;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Gonzalez, B Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Mol Genet & Microbiol, Microbiol Lab, Alameda 340,Casilla 114-D, Santiago, Chile Pontificia Univ Catolica Chile Alameda 340,Casilla 114-D Santiago Chile
Citazione:
P. Clement et al., "Molecular characterization of a deletion/duplication rearrangement in tfd genes from Ralstonia eutropha JMP134(pJP4) that improves growth on 3-chlorobenzoic acid but abolishes growth on 2,4-dichlorophenoxyacetic acid", MICROBI-SGM, 147, 2001, pp. 2141-2148

Abstract

Ralstonia eutropha JMP134(pJP4) is able to grow on minimal media containing the pollutants 3-chlorobenzoate (3-CB) or 2,4-dichlorophenoxyacetate (2,4-D). tfd genes from the 88 kb plasmid pJP4 encode enzymes involved in the degradation of these compounds. During growth of strain JMP134 in liquid medium containing 3-CB, a derivative strain harbouring a similar to 95 kb plasmid was isolated. This derivative, designated JMP134(pJP4-F3), had an improved ability to grow on 3-CB, but had lost the ability to grow on 2,4-D. Sequence analysis of pJP4-F3 indicated that the plasmid had undergone a deletion of similar to 16 kb, which included the tfdA-tfdS intergenic region, spanning the tfdA gene to a previously unreported IS1071 element. The loss of the tfdA gene explains the failure of the derivative to grow on 2,4-D. A similar to 23 kb duplication of the region spanning tfdR-tfdD(II)C(II)E(II)F(II)-tfdB(II)-tfdK-ISJP4-tfdT-tfdC(I)D(I)E(I)F(I)-tfdB(I), giving rise to a 51-kb-long inverted repeat, was also observed. The increase in gene copy number for the tfdCD(DC)EF gene cluster may provide an explanation for the derivative strain's improved growth on 3-CB. These observations are additional examples of the metabolic plasticity of R. eutropha JMP134, one of the more versatile pollutant-degrading bacteria.

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Documento generato il 11/07/20 alle ore 06:40:10