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Titolo:
Characterization of the CsfC and CsfD proteins involved in the biogenesis of CS5 pili from enterotoxigenic Escherichia coli
Autore:
Duthy, TG; Manning, PA; Heuzenroeder, MW;
Indirizzi:
Inst Med & Vet Sci, Infect Dis Labs, Adelaide, SA 5000, Australia Inst Med& Vet Sci Adelaide SA Australia 5000 delaide, SA 5000, Australia Univ Adelaide, Discipline Microbiol & Immunol, Dept Mol Biosci, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia 5005 ci, Adelaide, SA 5005, Australia AstraZeneca R&D Boston, Waltham, MA 02451 USA AstraZeneca R&D Boston Waltham MA USA 02451 Boston, Waltham, MA 02451 USA
Titolo Testata:
MICROBIAL PATHOGENESIS
fascicolo: 3, volume: 31, anno: 2001,
pagine: 115 - 129
SICI:
0882-4010(200109)31:3<115:COTCAC>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-BINDING DOMAIN; OUTER-MEMBRANE; FIMBRIAL SUBUNITS; FIBRILLAR SUBUNIT; CENTRAL AUSTRALIA; MINOR COMPONENTS; ANTIGEN; EXPRESSION; ADHESION; GENES;
Keywords:
enterotoxigenic E coli; pili; outer membrane protein; CsfC; CsfD; minor pilin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Heuzenroeder, MW Inst Med & Vet Sci, Infect Dis Labs, Adelaide, SA 5000, Australia Inst Med & Vet Sci Adelaide SA Australia 5000 Australia
Citazione:
T.G. Duthy et al., "Characterization of the CsfC and CsfD proteins involved in the biogenesis of CS5 pili from enterotoxigenic Escherichia coli", MICROB PATH, 31(3), 2001, pp. 115-129

Abstract

The region required for biosynthesis of CS5 pili consists of six csf genes, with csfA encoding the major subunit. In this study, we describe the characterization of two of the genes constituting the region, csfC and csfD, but also identify the true morphology of the CS5 pilus by high resolution electron microscopy. CsfD was shown to be essential in the initiation of CS5 pilus biogenesis, did not possess any chaperone-like activity for the major subunit, and was an integral minor component of the pilus structure. Studies on CsfD translocation across the outer membrane in Escherichia coli K-12 using a csfA mutant also showed that CsfD is likely to be the first pilin subunit assembled. A specific in-frame deletion in the csfC gene resulted inthe complete absence of cell surface CS5 pili and prevented the translocation of CsfA and CsfD pilins across the outer membrane. Specific cell localization studies showed an accumulation of CsfC in the outer membranes of E. coli K-12, while complementation experiments with homologous outer membraneassembly genes from CS1 and CFA/I pili systems were unable to restore assembly of CS5 pili. The CS5 pilus was shown to be a 2 nm flexible fibrillar structure, which adopted a predominantly open helical conformation under theelectron microscope.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:34:13