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Titolo:
Highly sensitive Taqman((R)) PCR detection of Puumala hantavirus
Autore:
Garin, D; Peyrefitte, C; Crance, JM; Le Faou, A; Jouan, A; Bouloy, M;
Indirizzi:
CRSSA Emile Parde, Unite Virol, F-38702 Grenoble, France CRSSA Emile Parde Grenoble France F-38702 irol, F-38702 Grenoble, France IMTSSA Pharo, F-13998 Marseille, France IMTSSA Pharo Marseille France F-13998 A Pharo, F-13998 Marseille, France CHU Nancy, Virol Lab, F-54035 Nancy, France CHU Nancy Nancy France F-54035 U Nancy, Virol Lab, F-54035 Nancy, France Inst Pasteur, Lab Bunyavirides, F-75724 Paris 15, France Inst Pasteur Paris France 15 Lab Bunyavirides, F-75724 Paris 15, France
Titolo Testata:
MICROBES AND INFECTION
fascicolo: 9, volume: 3, anno: 2001,
pagine: 739 - 745
SICI:
1286-4579(200107)3:9<739:HSTPDO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; C VIRUS GENOME; REAL-TIME PCR; HEMORRHAGIC-FEVER; RENAL SYNDROME; NEPHROPATHIA-EPIDEMICA; GENETIC IDENTIFICATION; QUANTITATION; DNA; BUNYAVIRIDAE;
Keywords:
Hantavirus; Puumala virus; RT-PCR; quantification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Garin, D CRSSA Emile Parde, Unite Virol, F-38702 Grenoble, France CRSSA Emile Parde Grenoble France F-38702 8702 Grenoble, France
Citazione:
D. Garin et al., "Highly sensitive Taqman((R)) PCR detection of Puumala hantavirus", MICROBES IN, 3(9), 2001, pp. 739-745

Abstract

An increasing number of clinical cases of Hantavirus infections have been reported from various regions in Asia, Europe and North America. Hantaviruses (family Bunyaviridae, genus Hantavirus) are enveloped and possess a single-stranded trisegmented RNA genome of negative polarity. Rodents or insectivores are natural hosts of hantaviruses and transmit the virus to humans chiefly by aerosolisation. These viruses are the causative agents of haemorrhagic fever with renal and pulmonary syndromes. In the northeast of France,Puumala hantavirus causes, every year, more than 150 mild forms of haemorrhagic fever with a renal syndrome known as nephropathia epidemica. Serological tests may lack sensitivity for diagnosing early stages of infection andvirus isolation is limited because it grows poorly in cell culture. Since reverse transcription (RT)-PCR amplification is an efficient method for detecting viral genomes in patient specimens, we developed an assay using a Taqman (R) probe and compared it with the classical RT-PCR amplification. To achieve this goal, a Puumala strain was grown in Vero E6 cells and RNA extracted from the culture supernatant. We found that the semi-nested RT-PCR detected a minimal amount of 300 TCID50 mL(-1), while the Taqman (R) PCR allowed detection of less than 10 TCID50 mL(-1) and provided a quantitative analysis. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:51:51