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Titolo:
Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the omp2 locus
Autore:
Cloeckaert, A; Verger, JM; Grayon, M; Paquet, JY; Garin-Bastuji, B; Foster, G; Godfroid, J;
Indirizzi:
INRA, Pathol Infect & Immunol Lab, F-37380 Nouzilly, France INRA Nouzilly France F-37380 ect & Immunol Lab, F-37380 Nouzilly, France Ctr Etud & Rech Vet & Agrochim, B-1180 Brussels, Belgium Ctr Etud & Rech Vet & Agrochim Brussels Belgium B-1180 Brussels, Belgium Agence Francaise Secur Sanitaire Aliments, Unite Zoonoses Bacteriennes, F-94703 Maisons Alfort, France Agence Francaise Secur Sanitaire Aliments Maisons Alfort France F-94703 SAC Vet Serv, Inverness IV2 4J2, Scotland SAC Vet Serv Inverness Scotland IV2 4J2 erv, Inverness IV2 4J2, Scotland
Titolo Testata:
MICROBES AND INFECTION
fascicolo: 9, volume: 3, anno: 2001,
pagine: 729 - 738
SICI:
1286-4579(200107)3:9<729:COBSIF>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER-MEMBRANE PROTEINS; POLYMERASE CHAIN-REACTION; SPECIES-SPECIFIC MARKERS; GENUS BRUCELLA; MOLECULAR CHARACTERIZATION; STRAINS; SEQUENCE; DIFFERENTIATION; MELITENSIS; ABORTUS;
Keywords:
Brucella; marine mammals; omp2a; omp2b; DNA polymorphism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Cloeckaert, A INRA, Pathol Aviaire & Parasitol Stn, F-37380 Nouzilly, France INRA Nouzilly France F-37380 Stn, F-37380 Nouzilly, France
Citazione:
A. Cloeckaert et al., "Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the omp2 locus", MICROBES IN, 3(9), 2001, pp. 729-738

Abstract

A number of recent reports have described the isolation and characterization of Brucella strains from a wide variety of marine mammals such as seals,porpoises, dolphins and a minke whale. These strains were identified as brucellae by conventional typing tests. However, their overall characteristics were not assimilable to those of any of the six currently recognized Brucella species and it was suggested that they comprise a new nomen species tobe called 'Brucella maris'. In the present study we analysed DNA polymorphism at the omp2 locus of 33 marine mammal Brucella strains isolated from seals, dolphins, porpoises and an otter. The omp2 locus contains two gene copies (named omp2a and omp2b) coding for porin proteins and has been found particularly useful for molecular typing and identification of Brucella at the species, biovar, or strain level. PCR-restriction fragment length polymorphism (RFLP) and DNA sequencing showed that strains isolated from dolphins and porpoises carry two omp2b gene copies instead of one omp2a and one omp2b gene copy or two similar omp2a gene copies reported in the currently recognized species. This observation was also recently made for a minke whale Brucella isolate. The otter and all seal isolates except one were shown to carry one omp2a and one omp2b gene copy as encountered in isolates from terrestrial mammals. By PCR-RFLP of the omp2b gene, a specific marker was detected grouping the marine mammal Brucella isolates. Although marine mammal Brucella isolates may represent a separate group from terrestrial mammal isolates based on omp2b sequence constructed phylogenetic trees, the divergencefound between their omp2b and also between their omp2a nucleotide sequences indicates that they form a more heterogeneous group than isolates from terrestrial mammals. Therefore, grouping the marine mammal Brucella isolates into one species 'Brucella maris' seems inappropriate unless the currently recognized Brucella species are grouped. With respect to the current classification of brucellae according to the preferential host, brucellae isolated from such diverse marine mammal species as seals and dolphins could actually comprise more than one species, and at least two new species, B. pinnipediae and B. cetaceae, could be compatible with the classical criteria of host preferentialism and DNA polymorphism at their omp2 locus. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 16:11:15