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Titolo:
Pressure-induced changes in the connectin/titin localization in the myofibrils revealed by immunoelectron microscopy
Autore:
Suzuki, A; Homma, Y; Kim, K; Ikeuchi, Y; Sugiyama, T; Saito, M;
Indirizzi:
Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata 9502181, Japan NiigataUniv Niigata Japan 9502181 ppl Biol Chem, Niigata 9502181, Japan Niigata Univ, Fac Agr, Dept Agr Sci, Niigata 9502181, Japan Niigata Univ Niigata Japan 9502181 Dept Agr Sci, Niigata 9502181, Japan Niigata Univ, Electron Microscop Lab, Niigata 9518510, Japan Niigata UnivNiigata Japan 9518510 Microscop Lab, Niigata 9518510, Japan
Titolo Testata:
MEAT SCIENCE
fascicolo: 2, volume: 59, anno: 2001,
pagine: 193 - 197
SICI:
0309-1740(200110)59:2<193:PCITCL>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH HYDROSTATIC-PRESSURE; POSTMORTEM BOVINE MUSCLE; SKELETAL-MUSCLE; ALPHA-CONNECTIN; ELASTIC PROTEIN; STRIATED-MUSCLE; BETA-CONNECTIN; TITIN; FILAMENTS; MEAT;
Keywords:
connectin; titin; monoclonal antibody; post-mortem muscle; high-pressure treatment;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, A Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata 9502181, Japan Niigata Univ Niigata Japan 9502181 hem, Niigata 9502181, Japan
Citazione:
A. Suzuki et al., "Pressure-induced changes in the connectin/titin localization in the myofibrils revealed by immunoelectron microscopy", MEAT SCI, 59(2), 2001, pp. 193-197

Abstract

Changes in the connectin/titin localization in post-mortem and pressurizedchicken muscles were investigated by immunoelectron microscopy. The anti-connectin monoclonal antibody, ID11, strongly labeled the sides of thick filaments near the H-zone and weakly labeled the sides of Z-line in the sarcomere prepared immediately after death. With the development of the muscle contraction, the shortening of the sarcomere and the dispersion of the connectin epitope near the H-zone were observed. With the gradual increase of thesarcomere length during further storage, the apparent increase of the width of the epitope in the A-band region stained by the antibody was observed,but the distance from the epitope to M-line remained almost the same length. In the case of high pressure treatment, significant changes in the labeling pattern of the antibody were observed with the increase of the pressureapplied. The increase of the distance from the epitope to M-line and dispersion of the epitope were observed in the fiber pressurized at 100 MPa. These phenomena were accelerated with the increase of the pressure applied. The discontinuous dense materials labeled by the antibody at the thick filament near the H-zone were observed in the fiber bundles pressurized at 200 MPa or more. This is probably due to the accumulation of connectin molecule from ordinary location in the sarcomere, because of the pressure-induced destruction of the thick and connectin filaments, In the fiber bundles pressurized at 300 MPa, a significant increase in the distance from the epitope toM-line accompanied with the increase of the sarcomere length was observed. From the results obtained, it was clear that the changes in the location of the connectin epitope induced by the brief exposure to high pressure weredrastic in comparison with that in the sarcomere during post-mortem storage. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 10:47:02