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Titolo:
Correlation between decreased apoptosis and multidrug resistance (MDR) in murine leukemic T cell lines
Autore:
Lopes, EC; Garcia, MG; Vellon, L; Alvarez, E; Hajos, SE;
Indirizzi:
Univ Buenos Aires, Fac Farm & Bioquim, Catedra Inmunol, IDEHU,CONICET, RA-1113 Buenos Aires, DF, Argentina Univ Buenos Aires Buenos Aires DF Argentina RA-1113 Aires, DF, Argentina
Titolo Testata:
LEUKEMIA & LYMPHOMA
fascicolo: 4, volume: 42, anno: 2001,
pagine: 775 -
SICI:
1042-8194(200108)42:4<775:CBDAAM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOCORTICOID-INDUCED APOPTOSIS; P-GLYCOPROTEIN; MOLECULAR MECHANISMS; ANTICANCER DRUGS; CYTOTOXIC DRUGS; DEATH; EXPRESSION; GENE; SENSITIVITY; ACTIVATION;
Keywords:
apoptosis; multidrug resistance; cross-resistance; antineoplasic drugs; leukemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Lopes, EC Univ Buenos Aires, Fac Farm & Bioquim, Catedra Inmunol, IDEHU,CONICET, Junin 956 4 Piso, RA-1113 Buenos Aires, DF, Argentina Univ Buenos Aires Junin 956 4 Piso Buenos Aires DF Argentina RA-1113
Citazione:
E.C. Lopes et al., "Correlation between decreased apoptosis and multidrug resistance (MDR) in murine leukemic T cell lines", LEUK LYMPH, 42(4), 2001, pp. 775

Abstract

Cancer cells may frequently develop cross-resistance to structurally dissimilar chemotherapeutic agents. However, the molecular mechanisms for sensitivity and resistance of tumor cells towards chemotherapy are still partially understood. Antineoplasic drugs have been shown to induce apoptosis in chemosensitive leukemias and solid tumors. In this work, cross-resistance among vincristine (VCR), doxorubicin (DOX) and other antineoplasic agents commonly used in the treatment of leukemia such as etoposide (VP-16), methotrexate (MTX), cyclophosphamide (CTX), dexamethasone (DEX), cytarabine (Ara-C) and L-asparaginase on vincristine resistant (LBR-V160), doxorubicin resistant (LBR-D160) and sensitive (LBR-) murine leukemic T cell lines, was determined. The effect of antineoplasic agents was assayed by tritiated thymidineincorporation. Our results showed that VCR exhibited cross-resistance withDOX, VP-16, DEX and MTX, while DOX demonstrated cross-resistance with VCR,VP-16 and MTX. Ara-C failed to present cross-resistance with any cell line. Apoptosis induced by the above drugs on the same cell lines was analyzed by acridine orange and ethidium bromide staining, DNA hypoploidy (flow cytometry) and oligonucleosomal fragmentation of nuclear DNA showing that therapeutic concentrations of these chemotherapeutic agents induced apoptosis inthe LBR- cell line. Our results demonstrated that, except for DEX, none ofthe drugs presenting cross-resistance were able to induce cell death on LBR-V160 or LBR-D160 cell lines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:40:02