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Titolo:
Ultrastructural features of CD34+ hematopoietic progenitor cells from bonemarrow, peripheral blood and umbilical cord blood
Autore:
Deliliers, GL; Caneva, L; Fumiatti, R; Servida, F; Rebulla, P; Lecchi, L; De Harven, E; Soligo, D;
Indirizzi:
Univ Milan, IRCCS, Ctr Trapianti Midollo, Osped Maggiore, I-20122 Milan, Italy Univ Milan Milan Italy I-20122 llo, Osped Maggiore, I-20122 Milan, Italy
Titolo Testata:
LEUKEMIA & LYMPHOMA
fascicolo: 4, volume: 42, anno: 2001,
pagine: 699 - 708
SICI:
1042-8194(200108)42:4<699:UFOCHP>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; STEM-CELLS; APOPTOSIS; CYCLE; BIOLOGY; STATE;
Keywords:
CD34+ hematopoietic stem cells; ultrastructure; myeloperoxidase; bone marrow; mobilized peripheral blood stem cells; cord blood;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Deliliers, GL Univ Milan, IRCCS, Ctr Trapianti Midollo, Osped Maggiore, Via Franceso Sforza 35, I-20122 Milan, Italy Univ Milan Via Franceso Sforza 35 Milan Italy I-20122 Italy
Citazione:
G.L. Deliliers et al., "Ultrastructural features of CD34+ hematopoietic progenitor cells from bonemarrow, peripheral blood and umbilical cord blood", LEUK LYMPH, 42(4), 2001, pp. 699-708

Abstract

Hematopoietic progenitor cells from different sources have been widely characterized, but their ultrastructural morphology has never been described in detail. In this study, imunomagnetically separated CD34(+) cells from normal bone marrow (BM), mobilized peripheral blood (PBSC) and human umbilicalcord blood (CB) were studied by transmission electron microscopy (TEM) using a cytochemical method which reveals endogenous myelo-peroxidase (MPO) activity. This technique is particularly suited for detecting early signs of the myeloid commitment. The CD34(+) cells from PBSC were morphologically very homogeneous and 94.7 +/- 4.5% of these cells were MPO-: these ultrastructural features are generally considered typical of immature cells. The CD34(+) BM cells were instead more heterogeneous, with 24.6 +/- 7.4% showing intense MPO activity. The ultrastructural characteristics of CB cells fell between those observed in PBSC and BM, but there was a high percentage of morphologically immature cells with no evidence of MPO activity (about 83%). The number of apoptotic cells within samples from different sources was alsoexamined both by TEM and flow cytometry. The percentage of apoptotic cellswas 0.7% in PBSC. 2.3% in BM, 2.9% in CB from vaginal delivery and 11.6% in CB from cesarean section. These observations confirm the relative phenotypic immaturity of CB in comparison with BM cells; they also suggest that CBcollected after cesarean section may be associated with reduced stem cellsviability.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 11:36:41