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Titolo:
T cell activation following infection of primary follicle center lymphoma B cells with adenovirus encoding CD154
Autore:
Cantwell, MJ; Wierda, WG; Lossos, IS; Levy, R; Kipps, TJ;
Indirizzi:
Univ Calif San Diego, Dept Med, Div Hematol Oncol, Sch Med, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 ch Med, La Jolla, CA 92093 USA Stanford Univ, Sch Med, Div Oncol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 ed, Div Oncol, Stanford, CA 94305 USA Immunogenex Inc, La Jolla, CA USA Immunogenex Inc La Jolla CA USAImmunogenex Inc, La Jolla, CA USA
Titolo Testata:
LEUKEMIA
fascicolo: 9, volume: 15, anno: 2001,
pagine: 1451 - 1457
SICI:
0887-6924(200109)15:9<1451:TCAFIO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC LYMPHOCYTIC-LEUKEMIA; SOLUBLE CD40 LIGAND; SYSTEMIC-LUPUS-ERYTHEMATOSUS; ANION-EXCHANGE HPLC; RECOMBINANT ADENOVIRUS; HYPER-IGM; IN-VIVO; EXPRESSION; ANTIGEN; GP39;
Keywords:
follicle center lymphoma; CD154; adenovirus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kipps, TJ Univ Calif San Diego, Dept Med, Div Hematol Oncol, Sch Med, 9500Gilman Dr, La Jolla, CA 92093 USA Univ Calif San Diego 9500 Gilman Dr La Jolla CA USA 92093 93 USA
Citazione:
M.J. Cantwell et al., "T cell activation following infection of primary follicle center lymphoma B cells with adenovirus encoding CD154", LEUKEMIA, 15(9), 2001, pp. 1451-1457

Abstract

Purified, high-titer adenovirus encoding murine CD154 (Ad-CD154) or human CD154 (Ad-hCD154) was used to infect lymph node cells isolated from patients with follicle center lymphoma. Infection of lymphoma B cells with Ad-CD154 at a multiplicity of infection (MOI) ratio of 100 or higher resulted in high-level transgene expression. Additionally, upon infection of lymphoma B cells, only Ad-CD154 resulted in surface expression of CD154, despite similar, high-level expression of either human or mouse CD154 by HeLa cells infected with Ad-hCD154 or Ad-CD154, respectively. Moreover, infection of lymphoma B cells with Ad-CD154, but not Ad-hCD154 or adenovirus encoding Eschericheria coli beta-galactosidase (Ad-LacZ), induced the neoplastic B cells toexpress higher levels of immune costimulatory molecules that are required for proficient presentation of antigen to T cells. Consistent with this, wefound that Ad-CD154 infected lymphoma B cells could stimulate T cells to proliferate or produce interferon-gamma in allogeneic or autologous mixed lymphocyte interactions. We conclude that lymphoma B cells can be infected with Ad-CD154 and that this significantly enhances their recognition by allogeneic or autologous T cells. As such, Ad-CD154-transduced lymphoma B cells may have potential for the active immune therapy of patients with follicle center lymphoma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:00:55