Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Induction of chemoresistance in HL-60 cells concomitantly causes a resistance to apoptosis and the synthesis of P-glycoprotein
Autore:
Campone, M; Vavasseur, F; Le Cabellec, MT; Meflah, K; Vallette, FM; Oliver, L;
Indirizzi:
INSERM, U419, F-44035 Nantes 01, France INSERM Nantes France 01INSERM, U419, F-44035 Nantes 01, France Ctr Reg Lutte Canc Nantes Atlantique, Nantes, France Ctr Reg Lutte Canc Nantes Atlantique Nantes France ique, Nantes, France
Titolo Testata:
LEUKEMIA
fascicolo: 9, volume: 15, anno: 2001,
pagine: 1377 - 1387
SICI:
0887-6924(200109)15:9<1377:IOCIHC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOCHROME-C RELEASE; ACUTE MYELOBLASTIC-LEUKEMIA; ACUTE MYELOID-LEUKEMIA; BCL-X-L; MULTIDRUG-RESISTANCE; HEMATOPOIETIC-CELLS; IN-VITRO; DEATH; EXPRESSION; BCL-X(L);
Keywords:
chemoresistance; P-glycoprotein; apoptosis; Bcl-XL; bcl-2; XIAP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Oliver, L INSERM, U419, IFR 26,9 Quai Moncousu, F-44035 Nantes 01, France INSERM IFR 26,9 Quai Moncousu Nantes France 01 antes 01, France
Citazione:
M. Campone et al., "Induction of chemoresistance in HL-60 cells concomitantly causes a resistance to apoptosis and the synthesis of P-glycoprotein", LEUKEMIA, 15(9), 2001, pp. 1377-1387

Abstract

The appearance of multidrug-resistant (MDR) proteins or the acquisition ofa defective apoptotic programme are major drawbacks in the treatment of cancers since both induce a resistance to classical chemotherapy. However, a link between the two mechanisms has not, as yet, been clearly established. In this study, HL-60 cells cultured in the continual presence of a sub-lethal dose of doxorubicin (dox; HL-60/Dox) were used as a model to study acquired chemoresistance. During the induction of chemoresistance, the appearance of a functional P-glycoprotein (P-gp), in addition to the expression of anti-apoptotic Bcl-2, Bcl-XL and pro-apoptotic Bax proteins was assessed. Parental cells which are sensitive to dox, have no P-gp activity and express Scl-2 and Bax. After 4 weeks of treatment, a functional P-gp was detected in HL-60/Dox cells. In addition, the synthesis of Bcl-2 appeared to be replaced by Bcl-XL while that of Bax remained unchanged. These cells were also resistant to apoptosis induced by both P-gp and non-P-gp substrates. This inability to induce apoptosis could have resulted from the induction of the expression of the inhibitor of apoptosis protein (XIAP). Our data show that acquired chemoresistance could involve a parallel induction of P-gp and an impairment of the apoptotic pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:29:37