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Titolo:
Response of Nicotiana alata to insertion of an autoregulated senescence-inhibition gene
Autore:
Schroeder, KR; Stimart, DP; Nordheim, EV;
Indirizzi:
Univ Wisconsin, Dept Hort, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 sin, Dept Hort, Madison, WI 53706 USA Univ Wisconsin, Dept Stat, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 sin, Dept Stat, Madison, WI 53706 USA
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
fascicolo: 5, volume: 126, anno: 2001,
pagine: 523 - 530
SICI:
0003-1062(200109)126:5<523:RONATI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC TOBACCO PLANTS; LEAF SENESCENCE; CYTOKININ; AGROBACTERIUM;
Keywords:
IPT; SAG; transgenics; transformation; leaf senescence; branching; flower longevity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Stimart, DP Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA Univ Wisconsin 1575 Linden Dr Madison WI USA 53706 I 53706 USA
Citazione:
K.R. Schroeder et al., "Response of Nicotiana alata to insertion of an autoregulated senescence-inhibition gene", J AM S HORT, 126(5), 2001, pp. 523-530

Abstract

Nicotiana alata Link and Otto (Jasmine tobacco) was transformed with an autoregulated senescence-inhibition gene construct P-SAGI2-IPT encoding isopentenyl transferase via Agrobacterium-mediated transformation. Transformation was confirmed by polymerase chain reaction. Transgenic plants exhibited up to 2- to 4-fold fewer senesced leaves, 29% longer in situ flower life, 26% more shoot dry weight, and a 32% to 50% reduction in flowers per branch. Additionally, transgenics were 28% shorter and had up to 174% more branches. indicative of cytokinin overproduction and a lack of tight autoregulationof P-SAG12-IPT. Variation among independent transgenics suggests selectionfor enhanced P-SAG12-IPT is feasible. Our observations of increased branching and in situ flower longevity, as well as reduced plant height and flowers per branch provide new information on P-SAG12-IPT and its potential value for biological study and horticultural application.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 10:59:25