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Titolo:
Neutral liposome-mediated delivery process of fluorescein-modified oligonucleotides in cultured human keratinocytes
Autore:
Sasaki, M; Hayashi, J; Fujii, M; Koizumi, K; Fujita, H; Kobayashi, M; Kawakubo, Y; Ito, A;
Indirizzi:
Tokai Univ, Sci & Technol Res Inst, Kanagawa 2591292, Japan Tokai Univ Kanagawa Japan 2591292 hnol Res Inst, Kanagawa 2591292, Japan Tokai Univ, Sch Engn, Dept Electrophotoopt, Kanagawa 2591292, Japan Tokai Univ Kanagawa Japan 2591292 ectrophotoopt, Kanagawa 2591292, Japan Tokai Univ, Sch Hlth Sci, Dept Nursing, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 Dept Nursing, Kanagawa 2591193, Japan Univ Tsukuba, Inst Mat Sci, Tsukuba, Ibaraki 3058753, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058753 sukuba, Ibaraki 3058753, Japan Tokai Univ, Sch Med, Dept Dermatol, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 Dept Dermatol, Kanagawa 2591193, Japan Tokai Univ, Sch Engn, Dept Nucl Engn, Hiratsuka, Kanagawa 2591292, Japan Tokai Univ Hiratsuka Kanagawa Japan 2591292 suka, Kanagawa 2591292, Japan
Titolo Testata:
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
fascicolo: 2-3, volume: 60, anno: 2001,
pagine: 120 - 128
SICI:
1011-1344(200107)60:2-3<120:NLDPOF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTISENSE OLIGONUCLEOTIDES; PHOSPHOROTHIOATE OLIGONUCLEOTIDES; CATIONIC LIPOSOMES; GENE INHIBITION; ANALOGS; DNA; OLIGODEOXYNUCLEOTIDES; METHYLPHOSPHONATES; DIFFERENTIATION; STABILITY;
Keywords:
antisense; oligonucleotide; keratinocyte; liposome; fluorescein; photo-induced binding;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Sasaki, M Tokai Univ, Sci & Technol Res Inst, 1117 Kitakaname, Kanagawa 2591292, Japan Tokai Univ 1117 Kitakaname Kanagawa Japan 2591292 591292, Japan
Citazione:
M. Sasaki et al., "Neutral liposome-mediated delivery process of fluorescein-modified oligonucleotides in cultured human keratinocytes", J PHOTOCH B, 60(2-3), 2001, pp. 120-128

Abstract

We propose a model of the intracellular delivery process in which fluorescein-labeled natural oligonucleotides (F-DNA) are transferred into the nuclei of cultured human keratinocytes. By encapsulation in neutral multilamellar lecithin liposomes, the F-DNA appeared to be protected against intracellular interactions with cellular materials and nuclease attacks in the cytoplasm during the process. The intracellular behavior of F-DNA and fluorescentphospholipid-labeled liposomes was observed by means of fluorescence analysis. Results showed that: F-DNA encapsulated in neutral multilamellar liposomes reached the cellular nuclei more efficiently than either free F-DNA, or F-DNA in unilamellar liposomes; the liposomal membranes appeared to be left in the cytoplasm. The reaction of F-DNA with complementary DNA was suggested by a rapid quenching of the fluorescence in the nucleus. In addition, the fluorescence decrease was evidently suppressed in the cytoplasm, indicating a protective effect of the neutral multilamellar liposomes against theinteraction of F-DNA with cytoplasmic materials. The application of these findings to 'photo'-antisense studies has been discussed, where suppressionof a gene expression is attempted by using oligonucleotide-attached fluorescein with the aid of a photo-induced covalent binding property. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 30/09/20 alle ore 00:37:06