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Titolo:
Determination of active component in silymarin by RP-LC and LC/MS
Autore:
Ding, TM; Tian, SJ; Zhang, ZX; Gu, DZ; Chen, YF; Shi, YH; Sun, ZP;
Indirizzi:
Hubei Inst Drug Control, Wuhan 430064, Peoples R China Hubei Inst Drug Control Wuhan Peoples R China 430064 64, Peoples R China Natl Inst Control Pharmaceut & Biol Prod, Div Instrumental Anal, Beijing 100050, Peoples R China Natl Inst Control Pharmaceut & Biol Prod Beijing Peoples R China 100050 China Pharmaceut Univ, Nanjing 210009, Peoples R China China Pharmaceut Univ Nanjing Peoples R China 210009 09, Peoples R China
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 1, volume: 26, anno: 2001,
pagine: 155 - 161
SICI:
0731-7085(200108)26:1<155:DOACIS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SILIBININ DIASTEREOMERS; PLASMA; ASSAY;
Keywords:
diastereoisomers; silymarin; LC/MS; RP-LC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Ding, TM Hubei Inst Drug Control, Wuhan 430064, Peoples R China Hubei InstDrug Control Wuhan Peoples R China 430064 es R China
Citazione:
T.M. Ding et al., "Determination of active component in silymarin by RP-LC and LC/MS", J PHARM B, 26(1), 2001, pp. 155-161

Abstract

Silybin, isosilybin, silydianin and silycristin in silymarin were separated and quantitatively determined by RP-HPLC. Two diastereoisomers of silybinand isosilybin were respectively separated by RP-HPLC and confirmed by LC/MS. Chromatographic condition consisted of column: Shim-pack VP-ODS (150 x 4.6 mm i.d. 5 mum) and Pre-column (10 x 4.6 mm i.d. 5 mum); mobile phase: methanol and solvent mixture (water: dioxane = 9:1) by gradient; flow rate: 1.5 ml/min; column temp.: 40 degreesC; detector wavelength: 288 nm; The recovery of 99.66% for silycristin, 99.48% for silydianin. 100.0% for silybin and 98.72% for isosilybin was respectively obtained. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:43:13