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Titolo:
Simultaneous determination of malathion, permethrin, DEET (N,N-diethyl-m-toluamide), and their metabolites in rat plasma and urine using high performance liquid chromatography
Autore:
Abu-Qare, AW; Abou-Donia, MB;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Pharmacol & Canc Biol, Durham, NC 27710 USA
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 2, volume: 26, anno: 2001,
pagine: 291 - 299
SICI:
0731-7085(200109)26:2<291:SDOMPD>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; INSECT REPELLENT N,N-DIETHYL-M-TOLUAMIDE; ORGANOPHOSPHORUS PESTICIDES; MASS-SPECTROMETRY; PYRETHROIDS; ABSORPTION; BLOOD; EXCRETION; SERUM; HPLC;
Keywords:
West Nile Virus; reversed phase HPLC; combined exposure; malathion; DEET; permethrin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, MB Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, POB 3813, Durham, NC 27710USA Duke Univ POB 3813 Durham NC USA 27710 Durham, NC 27710USA
Citazione:
A.W. Abu-Qare e M.B. Abou-Donia, "Simultaneous determination of malathion, permethrin, DEET (N,N-diethyl-m-toluamide), and their metabolites in rat plasma and urine using high performance liquid chromatography", J PHARM B, 26(2), 2001, pp. 291-299

Abstract

A method was developed for the separation and quantification of the insecticide malathion (O,O-dimethyl-S-(1,2-carbethoxyethyl) phosphorodithioate). its metabolite malaoxon (O,O-dimethyl-S-(1,2-carbethoxyethyl) phosphorothioate), the insecticide permethrin (3-(2,2-dichloro-ethenyl)-2,2-dimethylcyclopropanecarboxylic acid(3-phenoxyphenyl)methylester), two of its metabolites m-phenoxybenzyl alcohol and m-phenoxybenzoic acid, the insect repellent N,N-diethyl-m-toluamide (DEET), and its metabolites m-toluamide and in-toluic acid in rat plasma and urine. The method used high performance liquid chromatography (HPLC) with reversed phase C-18 column, and UV detection at 210nm. The compounds were separated using gradient of 45-99% acetonitrile in water (pH 3.5) at a flow rate ranging between 0.5 and 2 ml/min in a period of 15 min. The retention times ranged from 7.4 to 12.3 min. The limits of detection ranged between 20 and 100 ng/ml, while limits of quantitation were50-150 ng/ml. Average percentage recovery of five spiked plasma samples were 80.1 +/- 4.2, 75.2 +/- 4.6, 84.5 +/- 4.0, 84.3 +/- 3.4, 82.8 +/- 3.9, 83.9 +/- 5.5, 82.2 +/- 6.0, 83.1 +/- 4.3, and from urine 78.8 +/- 3.9, 76.4 +/- 4.9, 82.3 +/- 4.5, 82.5 +/- 3.9, 81.4 +/- 4.0, 83.9 +/- 4.3, 81.5 +/- 5.0, and 84.5 +/- 3.8 for, malathion, malaoxon, DEET, m-toluamide, ni-toluic acid, permethrin, m-phenoxybenzyl alcohol, and m-phenoxybenzoic acid, respectively. The method was reproducible and linear over range between 100 and 1000 ng/ml. This method was applied to analyze the above chemicals and metabolites following combined dermal administration in rats. (C) 2001 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 17:45:56