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Titolo:
Determination of depleted uranium, pyridostigmine bromide and its metabolite in plasma and urine following combined administration in rats
Autore:
Abu-Qare, AW; Abou-Donia, MB;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Pharmacol & Canc Biol, Durham, NC 27710 USA
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 2, volume: 26, anno: 2001,
pagine: 281 - 289
SICI:
0731-7085(200109)26:2<281:DODUPB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHARMACOKINETICS; SPECTROMETRY; EXPOSURE; PELLETS; DEET;
Keywords:
Gulf War veterans illness; depleted uranium; pyridostigmine bromides; anti-nerve agent;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, MB Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, POB 3813, Durham, NC 27710USA Duke Univ POB 3813 Durham NC USA 27710 Durham, NC 27710USA
Citazione:
A.W. Abu-Qare e M.B. Abou-Donia, "Determination of depleted uranium, pyridostigmine bromide and its metabolite in plasma and urine following combined administration in rats", J PHARM B, 26(2), 2001, pp. 281-289

Abstract

A simple and reliable method was developed for the quantification of depleted uranium, the anti nerve agent drug pyridostigmine bromide (PB;3-dimethylaminocarbonyloxy-N-methyl pyridinium bromide) and its metabolite N-methyl-3-hydroxypyridinium bromide in rat plasma and urine. The method involved using solid phase extraction and spectrophotometric determination of uranium,and high performance liquid chromatography (HPLC) with reversed phase C-18column, and UV detection at 280 nm for PB and its metabolite. Uranium was derivatized using dibenzoylmethane (DBM) then the absorbance was measured at 405 rim. PB and its metabolite were separated using a gradient of 1-40% acetonitrile in 0.1% triflouroacetic acid water solution (pH 3.2) at a flow rate of 0.8 ml/min in a period of 14 min. Limits of detection were 2 ng/ml for uranium and 50 ng/ml for PB and its metabolite. Limits of quantitation were between 10 and 100 ng/ml for uranium and the other two analytes, respectively. Average percentage recovery of five spiked plasma samples were 83.7 +/- 8.6. 76.8 +/- 6.7, 79.1 +/- 7.1, and from urine 82.7 +/- 8.6, 79.3 +/- 9.5 and 78.0 +/- 6.2, for depleted uranium. PB and N-methyl-3-hydroxypyridinium bromide, respectively. The relationship between peak areas and concentration was linear for standards between 100 and 1000 ng/ml for all three analytes. This method was applied to analyze the above chemicals and metabolites following combined administration in rats. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 08:20:52