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Titolo:
Allodynia and hyperalgesia produced by specific inhibition of spinal c-fosexpression: Lack of correlation with dynorphin content
Autore:
Ibrahim, MM; Mata, HP; Chawla, M; Lai, J; Porreca, F; Malan, TP;
Indirizzi:
Univ Arizona, Dept Anesthesiol, Tucson, AZ 85724 USA Univ Arizona Tucson AZ USA 85724 , Dept Anesthesiol, Tucson, AZ 85724 USA Univ Arizona, Grad Program Pharmacol & Toxicol, Tucson, AZ 85724 USA Univ Arizona Tucson AZ USA 85724 harmacol & Toxicol, Tucson, AZ 85724 USA Univ Arizona, Dept Pharmacol, Tucson, AZ 85724 USA Univ Arizona Tucson AZUSA 85724 na, Dept Pharmacol, Tucson, AZ 85724 USA
Titolo Testata:
JOURNAL OF PAIN
fascicolo: 4, volume: 2, anno: 2001,
pagine: 241 - 249
SICI:
1526-5900(200108)2:4<241:AAHPBS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT PRODYNORPHIN GENE; IMMEDIATE-EARLY GENES; INTRATHECAL NALOXONE; CORD; IMMUNOREACTIVITY; PROTEIN; PAIN; NOCICEPTION; ACTIVATION; INDUCTION;
Keywords:
Fos; transcription factors; dynorphin; opioid; allodynia; hyperalgesia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Malan, TP Univ Arizona, Dept Anesthesiol, POB 245114, Tucson, AZ 85724 USAUniv Arizona POB 245114 Tucson AZ USA 85724 Tucson, AZ 85724 USA
Citazione:
M.M. Ibrahim et al., "Allodynia and hyperalgesia produced by specific inhibition of spinal c-fosexpression: Lack of correlation with dynorphin content", J PAIN, 2(4), 2001, pp. 241-249

Abstract

Inhibition of spinal Fos expression increases formalin-induced nociceptionand decreases spinal prodynorphin messenger ribonucleic acid (mRNA), suggesting that Fos modulates nociception by inducing dynorphin synthesis. This study tests the hypothesis that Fos modulates sensitivity to other somatic stimuli, such that inhibition of Fos expression will result in tactile allodynia and thermal hyperalgesia. In addition, it correlates the somatosensory effects of inhibition of Fos expression with spinal dynorphin content. Antisense oligodeoxynucleotide (ODN) to c-fos mRNA was administered by intrathecal infusion. Tactile sensitivity was tested by probing the hindpaw with von Frey filaments. Thermal sensitivity was quantitated by using withdrawallatency to radiant heat. Two percent formalin was injected into the dorsalhindpaw, and flinches were quantitated. Fos was quantitated by counting immunoreactive cells. Dynorphin was measured by immunoassay. Intrathecal antisense, but not mismatch, ODN resulted in tactile allodynia, thermal hyperalgesia, and hyperalgesia to formalin-induced nociception. Antisense ODN decreased Fos-like immunoreactivity after formalin injection but did not alter Jun-like immunoreactivity. Antisense ODN had differing effects on spinal dynorphin content, depending an the method of administration. These experiments show a role of Fos in modulating somatosensory sensitivity and suggest that induction of dynorphin synthesis is not the sole mechanism by which Fosdoes so. (C) 2001 by the American Pain Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:21:56