Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Evidence that CFTR channels can regulate the open duration of other CFTR channels: Cooperativity
Autore:
Krouse, ME; Wine, JJ;
Indirizzi:
Stanford Univ, Cyst Fibrosis Res Lab, Stanford, CA 94035 USA Stanford Univ Stanford CA USA 94035 rosis Res Lab, Stanford, CA 94035 USA
Titolo Testata:
JOURNAL OF MEMBRANE BIOLOGY
fascicolo: 3, volume: 182, anno: 2001,
pagine: 223 - 232
SICI:
0022-2631(20010801)182:3<223:ETCCCR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSMEMBRANE CONDUCTANCE REGULATOR; AIRWAY EPITHELIAL-CELLS; CYSTIC-FIBROSIS GENE; PROTEIN-KINASE-C; CHLORIDE CHANNEL; ACETYLCHOLINE-RECEPTORS; EXPRESSION; CAMP; PHOSPHORYLATION; HYDROLYSIS;
Keywords:
Cystic fibrosis; channel gating; calu-3; binomial; open time;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Krouse, ME Stanford Univ, Cyst Fibrosis Res Lab, Jordan Hall 420, Stanford, CA 94035 USA Stanford Univ Jordan Hall 420 Stanford CA USA 94035 A 94035 USA
Citazione:
M.E. Krouse e J.J. Wine, "Evidence that CFTR channels can regulate the open duration of other CFTR channels: Cooperativity", J MEMBR BIO, 182(3), 2001, pp. 223-232

Abstract

CFTR channels mediate secretion and absorption in epithelia, and cystic fibrosis is caused by their malfunction. CFTR proteins are members of the ABCtransporter family and are complexly regulated by phosphorylation and nucleosides; they also influence other channel activity. Do CFTR molecules alsoinfluence one another? Cooperativity has been observed among other channels and has been suggested for CFTR. Therefore, we looked for evidence of cooperativity among CFTR channels using three independent approaches. All three methods provided evidence for cooperativity in CFTR gating. We estimated mean open times, independent of the number of channels in the patch, in multi-channel patches and showed that, on average, they increased as channel number increased. We observed many trials having larger than expected variances, consistent with cooperative gating. We also measured deviations from binomial statistics, which revealed cooperativity and further indicated thatits magnitude is underestimated to an unknown extent because of masking that occurs when CFTR channel populations within a single patch have heterogeneous open probabilities. Simulations showed that the observed departures from binomial statistics were too large to have arisen by chance. The evidence that CFTR P(o) increases with channel density has important functional implications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:43:27