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Titolo:
Controlling culture dynamics for the expansion of hematopoietic stem cells
Autore:
Madlambayan, GJ; Rogers, I; Casper, RF; Zandstra, PW;
Indirizzi:
Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada Univ Toronto Toronto ON Canada M5S 3G9 Engn, Toronto, ON M5S 3G9, Canada Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3G9, Canada UnivToronto Toronto ON Canada M5S 3G9 Chem, Toronto, ON M5S 3G9, Canada Univ Toronto, Dept Anat & Cell Biol, Toronto, ON M5S 3G9, Canada Univ Toronto Toronto ON Canada M5S 3G9 Biol, Toronto, ON M5S 3G9, Canada Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada Mt Sinai Hosp Toronto ON Canada M5G 1X5 Inst, Toronto, ON M5G 1X5, Canada
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 4, volume: 10, anno: 2001,
pagine: 481 - 492
SICI:
1525-8165(200108)10:4<481:CCDFTE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
UMBILICAL-CORD-BLOOD; EX-VIVO EXPANSION; GROWTH-FACTOR-BETA; IMMUNE-DEFICIENT MICE; BONE-MARROW CELLS; COMBINED IMMUNODEFICIENT MICE; COLONY-STIMULATING FACTOR; LONG-TERM HEMATOPOIESIS; C-KIT LIGAND; PROGENITOR CELLS;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
144
Recensione:
Indirizzi per estratti:
Indirizzo: Zandstra, PW Univ Toronto, Inst Biomat & Biomed Engn, Room 407,Roseburgh Bldg,4 Taddle Creek Rd, Toronto, ON M5S 3G9, Canada Univ Toronto Room 407,Roseburgh Bldg,4 Taddle Creek Rd Toronto ON Canada M5S 3G9
Citazione:
G.J. Madlambayan et al., "Controlling culture dynamics for the expansion of hematopoietic stem cells", J HEMATH ST, 10(4), 2001, pp. 481-492

Abstract

The ex vivo expansion of hematopoietic stem cells (HSCs) is the subject ofintense commercial and academic interest due to the potential of HSCs to be a renewable source of material for cellular therapeutics. Unfortunately, because methodologies have not yet been developed to grow clinically relevant numbers of HSCs (or their derivatives) consistently, the potential of this technology is limited. Manipulation of the in vitro culture microenvironment, primarily through cytokine supplementation, has been the predominant approach in studies attempting to expand primary human HSC numbers in vitro. While promising results have been obtained, it is becoming clear that novel methods must be developed before cellular therapies using these stem cells can become routine. Ideally, bioprocesses must be designed to target specifically the growth of stem cell populations while incorporating positive and negative feedback from potentially dynamic mature and maturing cell populations. The product of these culture systems should consist of not only HSCs, but also of cells that allow the engraftment of HSCs and, ideally, cells responsible for the immediate or accelerated functional support of patients. Development of such "designer transplants" will require combining optimal culture conditions capable of amplifying HSC numbers with novel approaches for finely controlling the number, functional capabilities, and characteristics of potentially therapeutic cells in these very complex cell culture systems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:05:31