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Titolo:
Isopropyl unoprostone increases the activities of matrix metalloproteinases in cultured monkey ciliary muscle cells
Autore:
Kashiwagi, K; Jin, M; Suzuki, M; Tanaka, Y; Iizuka, Y; Tsukahara, S;
Indirizzi:
Yamanashi Med Univ, Dept Ophthalmol, Tamaho, Yamanashi 4093898, Japan Yamanashi Med Univ Tamaho Yamanashi Japan 4093898 amanashi 4093898, Japan China Med Univ, Dept Ophthalmol, Shenyang, Peoples R China China Med UnivShenyang Peoples R China lmol, Shenyang, Peoples R China Corp Prod & Res Lab Primates, Tsukuba, Ibaraki, Japan Corp Prod & Res Lab Primates Tsukuba Ibaraki Japan ukuba, Ibaraki, Japan
Titolo Testata:
JOURNAL OF GLAUCOMA
fascicolo: 4, volume: 10, anno: 2001,
pagine: 271 - 276
SICI:
1057-0829(200108)10:4<271:IUITAO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRABECULAR MESHWORK CELLS; AQUEOUS-HUMOR DYNAMICS; OPHTHALMIC SOLUTIONS; UVEOSCLERAL OUTFLOW; TOPICAL APPLICATION; PROSTAGLANDINS; GLAUCOMA; UF-021; DEXAMETHASONE; MECHANISM;
Keywords:
ciliary muscle cells; isopropyl unoprostone; matrix metalloproteinases; prostaglandin F-2 alpha; trabecular meshwork cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kashiwagi, K Yamanashi Med Univ, Dept Ophthalmol, 1110 Shimokato, Tamaho, Yamanashi 4093898, Japan Yamanashi Med Univ 1110 Shimokato Tamaho YamanashiJapan 4093898
Citazione:
K. Kashiwagi et al., "Isopropyl unoprostone increases the activities of matrix metalloproteinases in cultured monkey ciliary muscle cells", J GLAUCOMA, 10(4), 2001, pp. 271-276

Abstract

Purpose: The mechanism by which the prostaglandin F-2 alpha-related antiglaucoma compound isopropyl unoprostone (refer-red to as unoprostone) reducesintraocular pressure is largely unknown. Another prostaglandin F-2 alpha-related compound, latanoprost. influences the activities of matrix metalloproteinases in ciliary muscle. Unoprostone ophthalmic solution is metabolizedto oxidized metabolites, mainly M1 and M2, in the eye. The aim of this study was to investigate whether intraocular metabolites of unoprostone, M1 and M2, change the metalloproteinase activity in cultured monkey ciliary muscle cells. Materials and Methods: Monkey ciliary muscle cells and trabecular meshworkcells were grown separately to confluence in monolayer cell cultures. M1 (10 nM, 100 nM, or 1 muM), M2 (10 nM, 100 nM, or 1 muM) 100 nM prostaglandinF-2 alpha, or vehicle solutions were added to each culture medium for 48 hours. The media were then assayed to measure metalloproteinase activities quantitatively by means of substrate zymography. Results: Compared with the vehicle controls, M1, M2. and prostaglandin F-2alpha Significantly increased the metalloproteinase-2 activity in culturedciliary muscle cells in a dose-dependent manner, but did not affect the metalloproteinase-2 activity in cultured trabecular meshwork cells. All experimented prostaglandins slightly increased metalloproteinase-9 activity in ciliary muscle cells, although these changes were not significant. Conclusions: The current results show that unoprostone influences the metabolism of the extracellular matrix in the ciliary muscle and that remodeling of the extracellular matrix in the ciliary muscle may be a possible mechanism by which unoprostone ophthalmic solution reduces intraocular pressure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:17:44