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Titolo:
Targeting of an expressed neurotoxin by its recombinant baculovirus
Autore:
Elazar, M; Levi, R; Zlotkin, E;
Indirizzi:
Hebrew Univ Jerusalem, Inst Life Sci, Dept Anim & Cell Biol, IL-91904 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91904 -91904 Jerusalem, Israel
Titolo Testata:
JOURNAL OF EXPERIMENTAL BIOLOGY
fascicolo: 15, volume: 204, anno: 2001,
pagine: 2637 - 2645
SICI:
0022-0949(200108)204:15<2637:TOAENB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSECT-SELECTIVE NEUROTOXIN; NUCLEAR POLYHEDROSIS-VIRUS; ANDROCTONUS-AUSTRALIS HECTOR; SCORPION-VENOM; POLYPEPTIDE; LARVAE; TOXIN; ANTIBODIES; INFECTION; TOLERANCE;
Keywords:
AaIT; CNS targeting; cooperativity; recombinant baculovirus; toxin potentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Zlotkin, E Hebrew Univ Jerusalem, Inst Life Sci, Dept Anim & Cell Biol, IL-91904 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91904 salem, Israel
Citazione:
M. Elazar et al., "Targeting of an expressed neurotoxin by its recombinant baculovirus", J EXP BIOL, 204(15), 2001, pp. 2637-2645

Abstract

AaIT, an insect-selective neurotoxic polypeptide derived from scorpion venom, has recently been used to engineer recombinant baculoviruses for insectpest control. Lepidopterous larvae infected with an AaIT-expressing baculovirus; reveal symptoms of paralysis identical to those induced by injectionof the native toxin. However, the paralyzed larvae treated by the recombinant virus possess an approximately 50-fold lower hemolymph toxin concentration than insects paralyzed by the native toxin. The mechanism of this potentiation effect was studied using immunocytochemistry, electrophysiology andtoxicity assays. (i) Light microscopy, using peroxidase-conjugated antibodies, revealed the presence of toxin in virus-susceptible tissues, includingtracheal epithelia located close to the central nervous system and beyond its lamellar enveloping sheath. (ii) High-resolution immunogold electron microscopical cytochemistry clearly revealed the presence of recombinant AaITtoxin inside the thoracic and abdominal ganglia on neuronal cell bodies and axonal membranes. (iii) Ventral nerve cords dissected from silkworm larvae infected with the recombinant baculovirus exhibited a high degree of excitability, expressed as enhanced frequency and bursting mode of their spontaneous activity, when compared to nerve cords infected with the wild-type virus. We conclude that the recombinant-virus-infected tracheal epithelia, outbranching in the body of an infected insect, (i) locally supply a continuous, freshly produced toxin to its neuronal receptors and (ii) introduce theexpressed toxin to the insect central nervous system, thus providing it with critical target sites that are inaccessible to the native toxin.

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Documento generato il 28/11/20 alle ore 14:14:23