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Titolo:
Lipopolysaccharide-induced apoptosis of macrophages determines the up-regulation of concentrative nucleoside transporters Cnt1 and Cnt2 through tumornecrosis factor-alpha-dependent and -independent mechanisms
Autore:
Soler, C; Valdes, R; Garcia-Manteiga, J; Xaus, J; Comalada, M; Casado, FJ; Modolell, M; Nicholson, B; MacLeod, C; Felipe, A; Celada, A; Pastor-Anglada, M;
Indirizzi:
Univ Barcelona, Dept Bioquim & Biol Mol, Regulat Transport Syst Grp, Fac Biol, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 ac Biol, E-08028 Barcelona, Spain Univ Barcelona, Dept Fisiol Biol Macrofag, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 acrofag, E-08028 Barcelona, Spain Max Planck Inst Immunbiol, D-79108 Freiburg, Germany Max Planck Inst Immunbiol Freiburg Germany D-79108 108 Freiburg, Germany Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 pt Med, La Jolla, CA 92093 USA Univ Calif San Diego, Ctr Canc, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 r Canc, La Jolla, CA 92093 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 32, volume: 276, anno: 2001,
pagine: 30043 - 30049
SICI:
0021-9258(20010810)276:32<30043:LAOMDT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOSINE-INDUCED APOPTOSIS; OXIDE SYNTHASE EXPRESSION; TNF-ALPHA; HL-60 CELLS; RAW-264.7 MACROPHAGES; MOLECULAR-BIOLOGY; IFN-GAMMA; RECEPTOR; RELEASE; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Pastor-Anglada, M Univ Barcelona, Dept Bioquim & Biol Mol, Regulat Transport Syst Grp, Fac Biol, Diagonal 645, E-08028 Barcelona, Spain Univ Barcelona Diagonal 645 Barcelona Spain E-08028 ain
Citazione:
C. Soler et al., "Lipopolysaccharide-induced apoptosis of macrophages determines the up-regulation of concentrative nucleoside transporters Cnt1 and Cnt2 through tumornecrosis factor-alpha-dependent and -independent mechanisms", J BIOL CHEM, 276(32), 2001, pp. 30043-30049

Abstract

In murine bone marrow macrophages, lipopolysaccharide (LPS) induces apoptosis through the autocrine production of tumor necrosis factor-alpha (TNF-alpha), as demonstrated by the fact that macrophages from TNF-alpha receptor I knock-out mice did not undergo early apoptosis. In these conditions LPS up-regulated the two concentrative high affinity nucleoside transporters here shown to be expressed in murine bone marrow macrophages, concentrative nucleoside transporter (CNT) 1 and 2, in a rapid manner that is nevertheless consistent with the de novo synthesis of carrier proteins. This effect was not dependent on the presence of macrophage colony-stimulating factor, although LPS blocked the macrophage colony-stimulating factor-mediated up-regulation of the equilibrative nucleoside transport system es. TNF-alpha mimicked the regulatory response of nucleoside transporters triggered by LPS, butmacrophages isolated from TNF-alpha receptor I knock-out mice similarly up-regulated nucleoside transport after LPS treatment. Although NO is produced by macrophages after LPS treatment, NO is not involved in these regulatory responses because LPS up-regulated CNT1 and CNT2 transport activity and expression in macrophages from inducible nitric oxide synthase and cationic amino acid transporter (CAT) 2 knock-out mice, both of which lack induciblenitric oxide synthesis. These data indicate that the early proapoptotic responses of macrophages, involving the up-regulation of CNT transporters, follow redundant regulatory pathways in which TNF-alpha- dependent- and -independent mechanisms are involved. These observations also support a role forCNT transporters in determining extracellular nucleoside availability and modulating macrophage apoptosis.

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Documento generato il 10/07/20 alle ore 03:01:24