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Titolo:
Recombinational and mutagenic repair of psoralen interstrand cross-links in Saccharomyces cerevisiae
Autore:
Greenberg, RB; Alberti, M; Hearst, JE; Chua, MA; Saffran, WA;
Indirizzi:
CUNY Queens Coll, Dept Chem & Biochem, Flushing, NY 11367 USA CUNY Queens Coll Flushing NY USA 11367 & Biochem, Flushing, NY 11367 USA Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Dept Chem, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 pt Chem, Berkeley, CA 94720 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 34, volume: 276, anno: 2001,
pagine: 31551 - 31560
SICI:
0021-9258(20010824)276:34<31551:RAMROP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE EXCISION-REPAIR; MAMMALIAN-CELL EXTRACTS; GENE CONVERSION TRACTS; DOUBLE-STRAND BREAKS; ESCHERICHIA-COLI; DNA-POLYMERASE; RECIPROCAL RECOMBINATION; 8-METHOXYPSORALEN PLUS; PLASMID RECOMBINATION; THYMINE DIMER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Saffran, WA CUNY Queens Coll, Dept Chem & Biochem, 65-30 Kissena Blvd, Flushing, NY 11367 USA CUNY Queens Coll 65-30 Kissena Blvd Flushing NY USA 11367 USA
Citazione:
R.B. Greenberg et al., "Recombinational and mutagenic repair of psoralen interstrand cross-links in Saccharomyces cerevisiae", J BIOL CHEM, 276(34), 2001, pp. 31551-31560

Abstract

Psoralen photoreacts with DNA to form interstrand cross-links, which can be repaired by both nonmutagenic nucleotide excision repair and recombinational repair pathways and by mutagenic pathways. In the yeast Saccharomyces cerevisiae, psoralen cross-links are processed by nucleotide excision repairto form double-strand breaks (DSBs). In yeast, DSBs are repaired primarilyby homologous recombination, predicting that cross-link and DSB repair should induce similar recombination end points. We compared psoralen cross-link, psoralen monoadduct, and DSB repair using plasmid substrates with site-specific lesions and measured the patterns of gene conversion, crossing over, and targeted mutation. Psoralen cross-link induced both recombination andmutations, whereas DSBs induced only recombination, and monoadducts were neither recombinogenic nor mutagenic. Although the cross-link- and DSB-induced patterns of plasmid integration and gene conversion were similar in mostrespects, they showed opposite asymmetries in their unidirectional conversion tracts: primarily upstream from the damage site for cross-links but downstream for DSBs. Cross-links induced targeted mutations in 5% of the repaired plasmids; all were base substitutions, primarily T --> C transitions. The major pathway of psoralen cross-link repair in yeast is error-free and involves the formation of DSB intermediates followed by homologous recombination. A fraction of the cross-links enter an error-prone pathway, resultingin mutations at the damage site.

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Documento generato il 08/04/20 alle ore 12:05:56