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Titolo:
Mechanism of inhibition of matrix metalloproteinase-9 induction by NO in vascular smooth muscle cells
Autore:
Gurjar, MV; DeLeon, J; Sharma, RV; Bhalla, RC;
Indirizzi:
Univ Iowa, Coll Med, Dept Anat & Cell Biol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 nat & Cell Biol, Iowa City, IA 52242 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 3, volume: 91, anno: 2001,
pagine: 1380 - 1386
SICI:
8750-7587(200109)91:3<1380:MOIOMM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED-PROTEIN-KINASE; SUPEROXIDE ANION PRODUCTION; NITRIC-OXIDE; CORONARY-ARTERIES; ANGIOTENSIN-II; FOAM CELLS; IN-VITRO; EXPRESSION; GROWTH; MIGRATION;
Keywords:
reactive oxygen species; matrix metalloproteinases; extracellular signal-regulated kinase; vascular smooth muscle cells; interleukin-1 beta; nitric oxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Bhalla, RC Univ Iowa, Coll Med, Dept Anat & Cell Biol, 1-564 BSB, Iowa City, IA 52242USA Univ Iowa 1-564 BSB Iowa City IA USA 52242 wa City, IA 52242USA
Citazione:
M.V. Gurjar et al., "Mechanism of inhibition of matrix metalloproteinase-9 induction by NO in vascular smooth muscle cells", J APP PHYSL, 91(3), 2001, pp. 1380-1386

Abstract

Vascular smooth muscle (VSM) cell migration is a critical step in the development of a neointima after angioplasty. Matrix metalloproteinases (MMPs) degrade the basement membrane and extracellular matrix, facilitating VSM cell migration. Recently, we demonstrated that nitric oxide (NO) inhibits interleukin-1 beta (IL-1 beta)-stimulated MMP-9 induction in rat aortic VSM cells. In this study, we examined the hypothesis that NO inhibits MMP-9 induction by attenuating superoxide generation and extracellular signal-regulated kinase (ERK) activation. Stimulation of VSM cells with IL-1 beta significantly (P<0.05) increased superoxide production, ERK activation, and MMP-9 induction. Pretreatment of VSM cells with the NO donor DETA NONOate significantly (P<0.05) decreased IL-1 beta -stimulated superoxide generation. In addition, pretreatment of VSM cells with a specific ERK pathway inhibitor, PD-98059, or DETA NONOate inhibited IL-1 beta -stimulated ERK activation and MMP-9 induction. Direct exposure of VSM cells to increased superoxide levels by treatment with xanthine/xanthine oxidase increased ERK activation and MMP-9 induction, whereas pretreatment of cells with PD-98059 significantly (P<0.05) inhibited xanthine/xanthine oxidase-stimulated ERK activation and MMP-9 induction. We conclude that NO inhibits IL-1<beta>-stimulated MMP-9 induction by inhibiting superoxide generation and subsequent ERK activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 07:43:12