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Titolo:
beta(1)-subunit of BK channels regulates arterial wall [Ca2+] and diameterin mouse cerebral arteries
Autore:
Lohn, M; Lauterbach, B; Haller, H; Pongs, O; Luft, FC; Gollasch, M;
Indirizzi:
Humboldt Univ, Charite Univ Hosp, Franz Volhard Clin, D-13125 Berlin, Germany Humboldt Univ Berlin Germany D-13125 lhard Clin, D-13125 Berlin, Germany Humboldt Univ, Charite Univ Hosp, Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany Humboldt Univ Berlin Germany D-13125 tr Mol Med, D-13125 Berlin, Germany Univ Hamburg, ZMNH, Inst Neuronale Signalverarbeitung, D-20246 Hamburg, Germany Univ Hamburg Hamburg Germany D-20246 arbeitung, D-20246 Hamburg, Germany Hannover Med Sch, Dept Nephrol, D-30625 Hannover, Germany Hannover Med Sch Hannover Germany D-30625 rol, D-30625 Hannover, Germany
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 3, volume: 91, anno: 2001,
pagine: 1350 - 1354
SICI:
8750-7587(200109)91:3<1350:BOBCRA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; INTRAVASCULAR PRESSURE; RYANODINE RECEPTORS; BETA-1 SUBUNIT; CALCIUM SPARKS; RAT; TONE;
Keywords:
calcium; calcium-activated potassium channels; pressurized cerebral arteries; arterial tone; BK beta(1) knockout mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Gollasch, M Franz Volhard Clin, Wiltbergstr 50, D-13125 Berlin, Germany Franz Volhard Clin Wiltbergstr 50 Berlin Germany D-13125 many
Citazione:
M. Lohn et al., "beta(1)-subunit of BK channels regulates arterial wall [Ca2+] and diameterin mouse cerebral arteries", J APP PHYSL, 91(3), 2001, pp. 1350-1354

Abstract

Mice with a disrupted beta (1) (BK beta (1))-subunit of the large-conductance Ca2+-activated K+ (BK) channel gene develop systemic hypertension and cardiac hypertrophy, which is likely caused by uncoupling of Ca2+ sparks to BK channels in arterial smooth muscle cells. However, little is known aboutthe physiological levels of global intracellular Ca2+ concentration ([Ca2+](i)) and its regulation by Ca2+ sparks and BK channel subunits. We utilized a BK beta (1) knockout C57BL/6 mouse model and studied the effects of inhibitors of ryanodine receptor and BK channels on the global [Ca2+](i) and diameter of small cerebral arteries pressurized to 60 mmHg. Ryanodine (10 muM) or iberiotoxin (100 nM) increased [Ca2+](i) by similar to 75 nM and constricted +/+ BK beta (1) wildtype arteries (pressurized to 60 mmHg) with myogenic tone by;10 mm. In contrast, ryanodine (10 mM) or iberiotoxin (100 nM)had no significant effect on [Ca2+](i) and diameter of -/- BK beta (1)-pressurized (60 mmHg) arteries. These results are consistent with the idea that Ca2+ sparks in arterial smooth muscle cells limit myogenic tone through activation of BK channels. The activation of BK channels by Ca2+ sparks reduces the voltage-dependent Ca2+ influx and [Ca2+](i) through tonic hyperpolarization. Deletion of BK beta (1) disrupts this negative feedback mechanism, leading to increased arterial tone through an increase in global [Ca2+](i).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:14:58