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Titolo:
Muscarinic excitation-contraction coupling mechanisms in tracheal and bronchial smooth muscles
Autore:
Janssen, LJ; Wattie, J; Lu-Chao, H; Tazzeo, T;
Indirizzi:
St Josephs Hosp, Asthma Res Grp, Firestone Inst Resp Hlth, Hamilton, ON L8N 4A6, Canada St Josephs Hosp Hamilton ON Canada L8N 4A6 , Hamilton, ON L8N 4A6, Canada McMaster Univ, Dept Med, Hamilton, ON L8N 3Z5, Canada McMaster Univ Hamilton ON Canada L8N 3Z5 ed, Hamilton, ON L8N 3Z5, Canada
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 3, volume: 91, anno: 2001,
pagine: 1142 - 1151
SICI:
8750-7587(200109)91:3<1142:MECMIT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACETYLCHOLINE-INDUCED CONTRACTIONS; CA2+ STORES; SIGNAL-TRANSDUCTION; DISTINCT PATHWAYS; CYTOSOLIC CALCIUM; SWINE TRACHEALIS; AIRWAY MYOCYTES; CANINE; RECEPTORS; CURRENTS;
Keywords:
airway smooth muscle contraction; airway hyperresponsiveness; Rho kinase; tyrosine kinase; phosphatidylinositol 3-kinase; calcium ion-adenosinetriphosphatase; genistein; calcium ion-dependent chlorine ion current;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Janssen, LJ St Josephs Hosp, Asthma Res Grp, Firestone Inst Resp Hlth, L-314,40 Charlton Ave E, Hamilton, ON L8N 4A6, Canada St Josephs Hosp L-314,40Charlton Ave E Hamilton ON Canada L8N 4A6
Citazione:
L.J. Janssen et al., "Muscarinic excitation-contraction coupling mechanisms in tracheal and bronchial smooth muscles", J APP PHYSL, 91(3), 2001, pp. 1142-1151

Abstract

We investigated the mechanisms underlying muscarinic excitation-contraction coupling in canine airway smooth muscle using organ bath, fura 2 fluorimetric, and patch-clamp techniques. Cyclopiazonic acid (CPA) augmented the responses to submaximal muscarinic stimulation in both tracheal (TSM) and bronchial smooth muscles (BSM), consistent with disruption of the barrier function of the sarcoplasmic reticulum. During maximal stimulation, however, CPA evoked substantial relaxation in TSM but not BSM. CPA reversal of carbachol tone persisted in the presence of tetraethylammoium or high KCl, suggesting that hyperpolarization is not involved; CPA relaxations were absent in tissues preconstricted with KCl alone or by permeabilization with beta -escin, ruling out a nonspecific effect on the contractile apparatus. Peak contractions were sensitive to inhibitors of tyrosine kinase (genistein) or Rhokinase (Y-27632). Sustained responses were dependent on Ca2+ influx in TSMbut not BSM; this influx was sensitive to Ni2+ but not La3+. In conclusion, there are several mechanisms underlying excitation-contraction coupling in airway smooth muscle, the relative importance of which varies depending on tissue and degree of stimulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 20:06:37