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Titolo:
Effect of mechanical deformation of neutrophils on their CD18/ICAM-1-dependent adhesion
Autore:
Anderson, GJ; Roswit, WT; Holtzman, MJ; Hogg, JC; Van Eeden, SF;
Indirizzi:
Univ British Columbia, Pulm Res Lab, St Pauls Hosp, Vancouver, BC V6Z 1Y6,Canada Univ British Columbia Vancouver BC Canada V6Z 1Y6 uver, BC V6Z 1Y6,Canada Washington Univ, Sch Med, Div Pulm, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 d, Div Pulm, St Louis, MO 63110 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 3, volume: 91, anno: 2001,
pagine: 1084 - 1090
SICI:
8750-7587(200109)91:3<1084:EOMDON>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
L-SELECTIN; RABBIT LUNGS; TRANSIT; DEFORMABILITY; CAPILLARIES; EXPRESSION; RETENTION; SURFACE; SEQUESTRATION; FILTERABILITY;
Keywords:
polymorphonuclear leukocytes; deformability; complement fragments; N-formyl-methionyl-leucyl-phenylalanine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Van Eeden, SF St Pauls Hosp, McDonald Res Lab, Vancouver, BC V6Z 1Y6, Canada St Pauls Hosp Vancouver BC Canada V6Z 1Y6 C V6Z 1Y6, Canada
Citazione:
G.J. Anderson et al., "Effect of mechanical deformation of neutrophils on their CD18/ICAM-1-dependent adhesion", J APP PHYSL, 91(3), 2001, pp. 1084-1090

Abstract

Mechanical deformation of polymorphonuclear leukocytes (PMN) changes theirexpression of the surface adhesion molecule CD11b/CD18. We tested the hypothesis that mechanical deformation of PMN enhances their adhesiveness. Purified human PMN were deformed through either 5- or 3-mum polycarbonate membrane filters and allowed to adhere to 96-well plates coated with human recombinant intercellular adhesion molecule-1 (ICAM-1). Flow cytometric studies showed that deformation of PMN increased CD11b/CD18 expression (P < 0.01). PMN adhesion to ICAM-1-coated plates was dependent on the magnitude of celldeformation (5 <mu>m, 63.8 +/- 8.1%, P < 0.04; 3 <mu>m, 232.4 +/- 20.9%, P< 0.01). Priming of PMN (0.5 nM N-formyl-methionyl-leucyl-phenylalanine) before deformation (5 <mu>m) increased PMN adhesion (63.8 +/- 8.1 vs. 105.3 /- 16.4%; P < 0.04). Stimulation (5% zymosan-activated plasma) of PMN after deformation resulted in increased adhesion, and the degree of increase was dependent on the magnitude of PMN deformation (stimulation, 50.6 +/- 4%; 5-<mu>m filtration and stimulation, 62.9 +/- 6.6%; 3-mum filtration and stimulation, 249.9 +/- 24.2%; P < 0.01). This study shows that mechanical deformation of PMN causes an increase in PMN adhesiveness to ICAM-1 that was enhanced by both priming of PMN before deformation and stimulation after celldeformation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:05:55