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Titolo:
Regulation of muscle GLUT-4 transcription by AMP-activated protein kinase
Autore:
Zheng, DH; MacLean, PS; Pohnert, SC; Knight, JB; Olson, AL; Winder, WW; Dohm, GL;
Indirizzi:
E Carolina Univ, Brody Sch Med, Dept Biochem, Greenville, NC 27858 USA E Carolina Univ Greenville NC USA 27858 Biochem, Greenville, NC 27858 USA Univ Oklahoma, Hlth Sci Ctr, Dept Biochem & Mol Biol, Oklahoma City, OK 73190 USA Univ Oklahoma Oklahoma City OK USA 73190 iol, Oklahoma City, OK 73190 USA Brigham Young Univ, Dept Zool, Provo, UT 84602 USA Brigham Young Univ Provo UT USA 84602 niv, Dept Zool, Provo, UT 84602 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 3, volume: 91, anno: 2001,
pagine: 1073 - 1083
SICI:
8750-7587(200109)91:3<1073:ROMGTB>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-TRANSPORTER GLUT4; DIABETIC TRANSGENIC MICE; RAT SKELETAL-MUSCLE; HIGH-FAT DIET; GENE-EXPRESSION; INSULIN SENSITIVITY; INCREASES GLUT-4; EXERCISE; TRANSLOCATION; RIBOSIDE;
Keywords:
myocyte enhancer factor-2; GLUT-4 promoter; transgenic mice; muscle fiber type;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Dohm, GL E Carolina Univ, Brody Sch Med, Dept Biochem, Greenville, NC 27858 USA E Carolina Univ Greenville NC USA 27858 Greenville, NC 27858 USA
Citazione:
D.H. Zheng et al., "Regulation of muscle GLUT-4 transcription by AMP-activated protein kinase", J APP PHYSL, 91(3), 2001, pp. 1073-1083

Abstract

Skeletal muscle GLUT-4 transcription in response to treatment with 5-aminoimidazole-4-carboxamide-1-beta -D-ribofuranoside (AICAR), a known activatorof AMP-activated protein kinase (AMPK), was studied in rats and mice. The increase in GLUT-4 mRNA levels in response to a single subcutaneous injection of AICAR, peaked at 13 h in white and red quadriceps muscles but not in the soleus muscle. The mRNA level of chloramphenicol acyltransferase reporter gene which is driven by 1,154 or 895 bp of the human GLUT-4 proximal promoter was increased in AICAR-treated transgenic mice, demonstrating the transcriptional upregulation of the GLUT-4 gene by AICAR. However, this induction of transcription was not apparent with 730 bp of the promoter. In addition, nuclear extracts from AICAR-treated mice bound to the consensus sequence of myocyte enhancer factor-2 (from -473 to -464) to a greater extent than from saline-injected mice. Thus AMP-activated protein kinase activation by AICAR increases GLUT-4 transcription by a mechanism that requires response elements within 895 bp of human GLUT-4 proximal promoter and that may be cooperatively mediated by myocyte enhancer factor-2.

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Documento generato il 04/04/20 alle ore 14:46:21