Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Influence of timing of administration of 5-fluorouracil to donors on bone marrow engraftment in nonmyeloablated hosts
Autore:
DHondt, L; Carlson, J; Benoit, B; Reilly, J; Grimaldi, C; Wuu, J; Lambert, JF; Dooner, MS; Quesenberry, PJ;
Indirizzi:
Ctr Hosp Notre Dame & Reine Fabiola, B-6000 Charleroi, Belgium Ctr Hosp Notre Dame & Reine Fabiola Charleroi Belgium B-6000 oi, Belgium Univ Massachusetts, Med Ctr, Ctr Canc, Worcester, MA USA Univ Massachusetts Worcester MA USA Med Ctr, Ctr Canc, Worcester, MA USA
Titolo Testata:
INTERNATIONAL JOURNAL OF HEMATOLOGY
fascicolo: 1, volume: 74, anno: 2001,
pagine: 79 - 85
SICI:
0925-5710(200107)74:1<79:IOTOAO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; MURINE MARROW; PROGENITOR CELLS; CYCLE STATUS; MICE; TRANSPLANTATION; LEADS; IL-11;
Keywords:
5-fluorouracil; hydroxyurea; hematopoietic stem cell; cell cycle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: D'Hondt, L Ctr Hosp Notre Dame & Reine Fabiola, Grand Rue 3, B-6000 Charleroi, Belgium Ctr Hosp Notre Dame & Reine Fabiola Grand Rue 3 Charleroi Belgium B-6000
Citazione:
L. D'Hondt et al., "Influence of timing of administration of 5-fluorouracil to donors on bone marrow engraftment in nonmyeloablated hosts", INT J HEMAT, 74(1), 2001, pp. 79-85

Abstract

We evaluated the engraftment and the cell cycle status of marrow cells at various times after 5-fluorouracil (5-FU) administration. 5-FU (150 mg/kg) was given to donor male BALB/c mice at 1, 2, 6, or 12 days prior to marrow harvest. The donor cells were then assessed in host nonmyeloablated female mice. Bone marrow engraftment of marrow treated with 5-FU was evaluated andcompared to marrow treated with diluent (phosphate-buffered saline) at 3 and 10 weeks after marrow infusion. Our data show a rapid induction of an engraftment defect I day after 5-FU, persistence of this defect through day 6, and a recovery by day 12. Experiments using hydroxyurea (which selectively kills cells in the S phase) to determine the cell cycle status indicated that cells that engrafted in post-5-FU marrow were noncycling at days 1, 2,and 12 but cycling at day 6. Post-5-FU bone marrow was also analyzed in vitro by colony assays and its cycling status determined by IH-thymidine suicide assay. High-proliferative-potential colony-forming cells (HPP-CFCs) andlow-proliferative-potential colony-forming cells (LPP-CFCs) decreased rapidly 1 day after 5-FU, with a nadir observed at day 6 for HPP-CFCs and day 2for LPP-CFCs. By day 12, LPP-CFCs showed a total recovery, but HPP-CFCs were still defective. Significant numbers of HPP-CFCs were cycling, mostly atdays 6 and 8 after 5-FU, whereas LPP-CFCs appeared quiescent except at day2. These results emphasize the importance of timing if post-5-FU marrow isused for gene therapy or marrow transplantation. (C) 2001 The Japanese Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:10:00