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Titolo:
Production of Neisseria meningitidis transferrin-binding protein B by recombinant Bordetella pertussis
Autore:
Coppens, I; Alonso, S; Antoine, R; Jacob-Dubuisson, F; Renauld-Mongenie, G; Jacobs, E; Locht, C;
Indirizzi:
Inst Pasteur, Lab Microbiol Genet & Mol, INSERM, U447, F-59019 Lille, France Inst Pasteur Lille France F-59019 l, INSERM, U447, F-59019 Lille, France Aventis Pasteur, F-69280 Marcy Letoile, France Aventis Pasteur Marcy Letoile France F-69280 69280 Marcy Letoile, France Transgene SA, F-67082 Strasbourg, France Transgene SA Strasbourg France F-67082 ne SA, F-67082 Strasbourg, France
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 9, volume: 69, anno: 2001,
pagine: 5440 - 5446
SICI:
0019-9567(200109)69:9<5440:PONMTP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE VESICLE VACCINE; FILAMENTOUS HEMAGGLUTININ; BACTERICIDAL ANTIBODIES; INTRANASAL IMMUNIZATION; SEQUENCE SIMILARITIES; HETEROLOGOUS ANTIGEN; IMMUNE-RESPONSES; ACCESSORY GENES; STRAINS; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Locht, C Inst Pasteur, Lab Microbiol Genet & Mol, INSERM, U447, 1 Rue ProfCalmette, F-59019 Lille, France Inst Pasteur 1 Rue Prof Calmette Lille France F-59019 le, France
Citazione:
I. Coppens et al., "Production of Neisseria meningitidis transferrin-binding protein B by recombinant Bordetella pertussis", INFEC IMMUN, 69(9), 2001, pp. 5440-5446

Abstract

Neisseria meningitidis serogroup B infections are among the major causes of fulminant septicemia and meningitis, especially severe in young children,and no broad vaccine is available yet. Because of poor immunogenicity of the serogroup B capsule, many efforts are now devoted to the identification of protective protein antigens. Among those are PorA and, more recently, transferrin-binding protein B (TbpB). In this study, TbpB of N. meningitidis was genetically fused to the N-terminal domain of the Bordetella pertussis filamentous hemagglutinin (FHA), and the fha-tbpB hybrid gene was expressedin A pertussis either as a plasmid-borne gene or as a single copy insertedinto the chromosome. The hybrid protein was efficiently secreted by the recombinant strains, despite its large size, and was recognized by both anti-FHA and anti-TbpB antibodies. A single intranasal administration of recombinant virulent or pertussis-toxin-deficient, attenuated B. pertussis to miceresulted in the production of antigen-specific systemic immunoglobulin G (IgG), as well as local IgG and IgA. The anti-TbpB serum antibodies were of the IgG1, IgG2a, and IgG2b isotypes and were found to express complement-mediated bactericidal activity against N. meningitidis. These observations indicate that recombinant B. pertussis may be a promising vector for the development of a mucosal vaccine against serogroup B meningococci.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:07:29