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Titolo:
Type 3 fimbrial shaft (MrkA) of Klebsiella pneumoniae, but not the fimbrial adhesin (MrkD), facilitates biofilm formation
Autore:
Langstraat, J; Bohse, M; Clegg, S;
Indirizzi:
Univ Iowa, Coll Med, Dept Microbiol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Dept Microbiol, Iowa City, IA 52242 USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 9, volume: 69, anno: 2001,
pagine: 5805 - 5812
SICI:
0019-9567(200109)69:9<5805:T3FS(O>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-AERUGINOSA; BACTERIAL BIOFILMS; ESCHERICHIA-COLI; MR/K FIMBRIAE; ADHERENCE; STRAINS; ENTEROBACTERIACEAE; DETERMINANTS; EXPRESSION; INSERTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Clegg, S Univ Iowa, Coll Med, Dept Microbiol, Iowa City, IA 52242 USA UnivIowa Iowa City IA USA 52242 robiol, Iowa City, IA 52242 USA
Citazione:
J. Langstraat et al., "Type 3 fimbrial shaft (MrkA) of Klebsiella pneumoniae, but not the fimbrial adhesin (MrkD), facilitates biofilm formation", INFEC IMMUN, 69(9), 2001, pp. 5805-5812

Abstract

Isolates of Klebsiella pneumoniae are responsible for opportunistic infections, particularly of the urinary tract and respiratory tract, in humans. These bacteria express type 3 fimbriae that have been implicated in binding to eucaryotic cells and matrix proteins. The type 3 fimbriae mediate binding to target tissue using the MrkD adhesin that is associated with the fimbrial shaft comprised of the MrkA protein. The formation of biofilms in vitroby strains of K. pneumoniae was shown to be affected by the production of fimbriae on the bacterial surface. However, a functional MrkD adhesin was not necessary for efficient biofilm formation. Nonfimbriate strains were impaired in their ability to form biofilms. Using isogenic fimbriate and nonfimbriate strains of K. pneumoniae expressing green fluorescent protein it was possible to demonstrate that the presence of type 3 fimbriae facilitated the formation of dense biofilms in a continuous-flowthrough chamber. Transformation of nonfimbriate mutants with a plasmid possessing an intact mrk gene cluster restored the fimbrial phenotype and the rapid ability to form biofilms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:30:33