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Titolo:
Nitric oxide attenuates the expression of transforming growth factor-beta(3) mRNA in rat cardiac fibroblasts via destabilization
Autore:
Abdelaziz, N; Colombo, F; Mercier, I; Calderone, A;
Indirizzi:
Inst Cardiol Montreal, Montreal, PQ H1T 1C8, Canada Inst Cardiol MontrealMontreal PQ Canada H1T 1C8 real, PQ H1T 1C8, Canada Univ Montreal, Dept Physiol, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 ol, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
HYPERTENSION
fascicolo: 2, volume: 38, anno: 2001,
pagine: 261 - 266
SICI:
0194-911X(200108)38:2<261:NOATEO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; ATRIAL-NATRIURETIC-PEPTIDE; CONGESTIVE-HEART-FAILURE; RECEPTOR MESSENGER-RNA; EXTRACELLULAR-MATRIX; FACTOR-BETA; 3'-UNTRANSLATED REGION; MOLECULAR-CLONING; GUANYLATE-CYCLASE; LUNG FIBROBLASTS;
Keywords:
nitric oxide; cyclic GMP; fibroblasts; RNA, messenger; transforming growth factors; angiotensin II;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Calderone, A Inst Cardiol Montreal, 5000 Rue Belanger Est, Montreal, PQ H1T 1C8, Canada Inst Cardiol Montreal 5000 Rue Belanger Est Montreal PQ Canada H1T 1C8
Citazione:
N. Abdelaziz et al., "Nitric oxide attenuates the expression of transforming growth factor-beta(3) mRNA in rat cardiac fibroblasts via destabilization", HYPERTENSIO, 38(2), 2001, pp. 261-266

Abstract

Transforming growth factor-beta (TGF-beta) has been implicated in the development of interstitial fibrosis in cardiac hypertrophy. NO has been regarded as a potent inhibitor of cardiac fibroblast growth, albeit the modulation of cellular events associated with interstitial fibrosis remains undefined. In this regard, the regulation of TGF-beta mRNA expression by the NO donor S-nitroso-N-acetyl-penicillamine (SNAP) was examined in neonatal rat cardiac fibroblasts. SNAP treatment for 4 hours decreased TGF-beta (3) mRNA levels, an effect mimicked by 8-bromo-cGMP. TGF-beta (3) mRNA, however, had returned to levels observed in the untreated cells after a 24-hour exposure to SNAP, whereas a decreased expression persisted with 8-bromo-cGMP. In contrast to TGF-beta (3), TGF-beta (1) mRNA levels were modestly increased in response to cGMP-generating molecules. The treatment with actinomycin D forat least 8 hours did not appreciably alter TGF-beta (3) mRNA levels. By contrast, SNAP treatment caused a rapid decrease of TGF-beta (3) mRNA with a half-life of 3.3 +/-0.2 hours, thereby supporting a mechanism of destabilization. The pretreatment with SNAP inhibited angiotensin II-stimulated protein synthesis and the concomitant expression of TGF-beta (3) mRNA. These data reveal a disparate pattern of TGF-beta (1) and TGF-beta (3) mRNA regulation by NO and highlight a novel mechanism of destabilization contributing tothe decreased expression of TGF-beta (3) mRNA. The modulation of both basal and angiotensin II-stimulated TGF-beta (3) mRNA expression provides a mechanism by which NO may influence the progression of interstitial fibrosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 12:59:22