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Titolo:
No influence of prednisolone on IGFBP-3 proteolysis in healthy young men
Autore:
Dall, R; Wolthers, T; Flyvbjerg, A; Grofte, T; Christiansen, JS; Jorgensen, JO;
Indirizzi:
Aarhus Kommune Hosp, Med Dept M, DK-8000 Aarhus C, Denmark Aarhus Kommune Hosp Aarhus Denmark C d Dept M, DK-8000 Aarhus C, Denmark Aarhus Kommune Hosp, Med Dept 5, Aarhus C, Denmark Aarhus Kommune Hosp Aarhus Denmark C Hosp, Med Dept 5, Aarhus C, Denmark Univ Aarhus, Inst Expt Clin Res, DK-8000 Aarhus C, Denmark Univ Aarhus Aarhus Denmark C st Expt Clin Res, DK-8000 Aarhus C, Denmark
Titolo Testata:
HORMONE RESEARCH
fascicolo: 2, volume: 55, anno: 2001,
pagine: 95 - 101
SICI:
0301-0163(2001)55:2<95:NIOPOI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-I; BINDING PROTEIN-3 PROTEOLYSIS; NORMAL-MALE VOLUNTEERS; HORMONE GH SECRETION; DEXAMETHASONE TREATMENT; FACTOR IGF; CUSHINGS-SYNDROME; FACTOR (IGF)-I; INSULIN; SERUM;
Keywords:
insulin-like growth factor I; glucocorticoid; insulin-like growth factor binding proteins; IGFBP-3 proteolysis; Western ligand blotting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Dall, R Aarhus Kommune Hosp, Med Dept M, DK-8000 Aarhus C, Denmark Aarhus Kommune Hosp Aarhus Denmark C , DK-8000 Aarhus C, Denmark
Citazione:
R. Dall et al., "No influence of prednisolone on IGFBP-3 proteolysis in healthy young men", HORMONE RES, 55(2), 2001, pp. 95-101

Abstract

Aims: The impact of growth hormone (GH) and prednisolone on the GH/insulin-like growth factor (IGF) axis with special emphasis on IGF binding protein-3 (IGFBP-3) proteolysis was studied in 8 healthy adults in a double-blind cross-over study with four periods: (1) placebo; (2) s.c. GH 0.1 IU/kg/day;(3) oral prednisolone 50 mg/day, and (4) co-administration of GH and prednisolone. Methods: Each treatment period lasted for 4 days followed by a washout period of 10 days. We measured IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3 by immunoassays, IGFBP-3 by Western ligand blotting (WLB) and finally in vitro IGFBP-3 proteolysis by a I-125-IGFBP-3 degradation assay. Results: IGF-I levels increased by 99% during GH administration and 67% during co-administration of GH and prednisolone (p < 0.0005), whereas no significant change was seen during prednisolone alone. IGFBP-1 levels decreased 55% during the prednisolone period (p < 0.002), but the between period changes were not significant (p < 0.1). IGFBP-2 decreased 33% during coadministration of GH and prednisolone (p < 0.002). IGFBP-3 increased 12% during GH and 7% during coadministration of GH and prednisolone (p < 0.003 and p < 0.03 comparedto placebo, respectively), whereas prednisolone alone induced no significant changes. IGFBP-3 measured by WLB did not change significantly, neither did IGFBP-3 proteolysis. Conclusions: Prednisolone administration induces only minimal changes in circulating components of the IGF axis and is not accompanied by alterations in IGFBP-3 proteolysis. This indicates that the metabolic effects of glucocorticoids do not depend on serum IGF-I. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 09:41:17