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Titolo:
Endothelial nitric oxide synthase is strongly expressed in malignant mesothelioma but does not associate with vascular density or the expression of VEGF, FLK1 or FLT1
Autore:
Soini, Y; Puhakka, A; Kahlos, K; Saily, M; Paakko, P; Koistinen, P; Kinnula, V;
Indirizzi:
Univ Oulu, Dept Pathol, FIN-90014 Oulu, Finland Univ Oulu Oulu Finland FIN-90014 u, Dept Pathol, FIN-90014 Oulu, Finland Univ Oulu, Dept Internal Med, FIN-90014 Oulu, Finland Univ Oulu Oulu Finland FIN-90014 t Internal Med, FIN-90014 Oulu, Finland Oulu Univ Hosp, Oulu, Finland Oulu Univ Hosp Oulu FinlandOulu Univ Hosp, Oulu, Finland
Titolo Testata:
HISTOPATHOLOGY
fascicolo: 2, volume: 39, anno: 2001,
pagine: 179 - 186
SICI:
0309-0167(200108)39:2<179:ENOSIS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR VEGF; HUMAN LUNG-CANCER; TUMOR-GROWTH; PLEURAL MESOTHELIOMA; TYROSINE KINASE; CELLS; ANGIOGENESIS; RECEPTOR; ADENOCARCINOMA; INVASIVENESS;
Keywords:
mesothelioma; angiogenesis; nitric oxide synthase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Soini, Y Univ Oulu, Dept Pathol, POB 5000,Aapistie 5, FIN-90014 Oulu, Finland Univ Oulu POB 5000,Aapistie 5 Oulu Finland FIN-90014 lu, Finland
Citazione:
Y. Soini et al., "Endothelial nitric oxide synthase is strongly expressed in malignant mesothelioma but does not associate with vascular density or the expression of VEGF, FLK1 or FLT1", HISTOPATHOL, 39(2), 2001, pp. 179-186

Abstract

Aims: To investigate endothelial nitric oxide synthase (eNOS) expression in malignant mesothelioma and its association with expression of vascular endothelial growth factor (VEGF), its receptors FLK1 and FLT1, and vascular density. Methods and results: eNOS, VEGF, ELK1 and FLT1 were studied in 36 histological mesothelioma samples by immunohistochemistry. Two mesothelioma (M14K, M38K) and one non-neoplastic mesothelial cell line (MET-5A) were studied for eNOS mRNA expression by reverse transcriptase-polymerase chain reaction (RT-PCR). Vascular density was determined by staining the samples with an antibody to factor VIII. RT-PCR showed that mesothelioma cells synthesize eNOS in vitro. eNOS immunoreactivity was found in 32/36 (89%) tumours. VEGF, FLK1 and FLT1 expression was found in 17 (45%), 24 (69%) and 25 (71%) cases,respectively. FLK1 or FLT1 immunoreactivity was more often seen in epithelioid and biphasic mesotheliomas than in sarcomatoid ones (P = 0.007 and P =0.011, respectively). There was a significant association between FLK1 andFLT1 immunoreactivity (P = 0.032). No significant association was found between FLK1, FLT1, VEGF and eNOS immunoreactivity and vascular density. Conclusions: eNOS is strongly expressed in malignant mesothelioma. Since eNOS did not associate with VEGF, FLK1 or FLT1, its synthesis seems not to be regulated through VEGF in malignant mesothelioma as has been shown in non-neoplastic endothelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:44:19